biostudies-arrayexpressKatie StevensSolanum tuberosumhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16611The experiment aimed to determine if potato and P infestans effectively process exogenous dsRNA. Both whole potato plants and in vitro P infestans were treated with 10 µg of dsRNA. Total RNA was extracted with the mirVana™ miRNA Isolation Kit. Library preparation and sequencing was conducted by NGI Sweden, SciLifeLab using a NovaSeq 6000 SP-100 flowcell. sRNA sequencing analysis demonstrated that both plant and pathogen effectively process target dsRNA into sRNAs, producing distinct populations of sRNA.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted with the mirVana miRNA isolation kit.Sequencing - Samples were sequenced on NovaSeq6000 (NovaSeq Control Software 1.8.1/RTA v3.4.4) with a 101nt(Read1)-8nt(Index1)-8nt(Index2) setup using 'NovaSeqStandard' workflow in 'SP' mode flowcell.Sample Collection - For P infestans, following a 12 hour incubation of dsRNA samples were collected by flash freezing micro eppendorf tubes. For plant samples, 48 hours post dsRNA spraying leaf samples were selected and flash frozen.Library Construction - Library preparation and sequencing was conducted by NGI Sweden, SciLifeLab using a NovaSeq 6000 SP-100 flowcell.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - fastp was used to remove adapter sequences and obtain reads 16-30 base per in length, duplicate reads were removed using umi tools [27, 28]. Reads were uniquely aligned to the PITG_06376 target gene using ShortStack (v 4.0.3) with default parameters [29]; normalised coverage of reads was calculated using Samtools [30]. For whole genome alignments, reads mapping to ribosomal RNA, transfer RNA and both the potato reference genome (https://spuddb.uga.edu/) and P. infestans genome (https://protists.ensembl.org/index.html) were removed before alignment and counting using Shortstack.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000microRNA profiling by high throughput sequencingSolanum tuberosumKatie StevensfalseSpray-Induced Gene Silencing (SIGS) with dsRNA: Efficacy, stability, and processing in potato plant protection against Phytophthora infestansThe experiment aimed to determine if potato and P infestans effectively process exogenous dsRNA. Both whole potato plants and in vitro P infestans were treated with 10 µg of dsRNA. Total RNA was extracted with the mirVana™ miRNA Isolation Kit. Library preparation and sequencing was conducted by NGI Sweden, SciLifeLab using a NovaSeq 6000 SP-100 flowcell. sRNA sequencing analysis demonstrated that both plant and pathogen effectively process target dsRNA into sRNAs, producing distinct populations of sRNA.2026-03-31T00:00:00Z2026-03-31T07:47:54.044Z2026-02-09T13:24:37.524ZE-MTAB-16611ERP188889EFO_0002944EFO_0004170EFO_0002896EFO_0005518EFO_0003816EFO_0004184