biostudies-arrayexpressDomenico PalumboHomo sapiensDESeq2https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16622CD146⁺ BMSCs were isolated from 10 human subjects, stratified into middle-aged (MA) and older adults (OA).biostudies-arrayexpressSample Collection - BM cells were isolated from femoral heads, otherwise discarded as surgical waste, obtained upon written informed consent from subjects undergoing hip replacement surgery due to coxarthrosis.Sequencing - Libraries were sequenced at a final concentration of 1.3 pM in paired-end mode (2 × 100 bp) on a NovaSeq 6000 platform (Illumina Inc.).Nucleic Acid Extraction - CD146⁺ BMSCs from each subject were plated at a density of 1.5 × 10⁵ cells per well in 6-well plates. After 3 days of incubation the total RNA was isolated using the miRNeasy Kit (QIAGEN, Hilden, Germany; cat # 217084), including an on-column DNase digestion step. RNA was subsequently precipitated with ethanol to further improve purity.Library Construction - Sequencing libraries were generated from 400 ng of total RNA as input with the TruSeq Stranded Total RNA kit (Illumina), according to the manufacturer’s instruction.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Reads were demultiplexed using bcl2fastq (v2.20.0.422; Illumina, SCR-015058), and read quality was assessed employing FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Adapter trimming was performed using cutadapt (v3.3). Cleaned reads were aligned to the human reference genome (hg38) using STAR (v2.7.11b). Gene expression quantification was carried out with featureCounts (v2.0.1), using the GENCODE v46 primary assembly annotation. Transcripts supported by more than 10 raw counts were considered expressed. Differential expression analysis was conducted in R using the DESeq2 package (v1.46.0) with default settings, retaining transcripts with an absolute fold change ≥ 1.5 and an adjusted p-value ≤ 0.05.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsTruSeq Stranded Total RNA kit (Illumina)Illumina NovaSeq 6000RNA-seq of total RNAHomo sapiensDomenico PalumbofalseImpact of Aging on CD146+ Mesenchymal Stromal Cells-Mediated Regulation of Bone Marrow CD34⁺ Hematopoietic Stem/Progenitor Cell MobilizationCD146⁺ BMSCs were isolated from 10 human subjects, stratified into middle-aged (MA) and older adults (OA).2026-04-29T00:00:00Z2026-04-29T01:01:54.677Z2026-02-10T12:49:41.155ZE-MTAB-16622ERP188939EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184