biostudies-arrayexpressJan KubovčiakHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16634The goal is to identify differntially expressed genes in multifocal osteomyelitis (CRMO) neutrophils to understand the mechanism of neutrophil dysregulation contributing to CRMO development.biostudies-arrayexpressSample Collection - Isolation of neutrophils was performed according to the protocol dx.doi.org/10.17504/protocols.io.dm6gpzq51lzp/v1 from approx. 7-9 ml of blood sampleLibrary Construction - Libraries were prepared with SMART-seq total RNA single cell (ZapR mammalian, 634360) kit according to manufacturer's instructions.Nucleic Acid Extraction - Isolation of total RNA from approx. 1-2 ml neutrophil solution was performed using R1055 Quick-RNA Miniprep Kit https://zymoresearch.eu/products/quick-rna-miniprep-kit according to manufacturer instruction, RNA was eluted with 50μl of Nuclease free water.Sequencing - Libraries were sequenced using the Illumina NextSeq® 2000 instrument using a P2 100 X-LEAP SBS kit with cDNA read length of 122 nt (single-end)OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - For subsequent read processing, a bioinformatic pipeline nf-core/rnaseq version 3.17 was used. Individual steps included removing sequencing adaptors and low-quality reads with Trim Galore!, mapping to reference genome GRCh38 (Ensembl annotation version 113) with STAR and quantifying expression on gene level based on mapped fragments with Salmon.Data Transformation - Reads were mapped to reference genome GRCh38 (Ensembl annotation version 113) with STAR and expression on gene level was quantified based on mapped fragments with Salmon.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000RNA-seq of coding RNAHomo sapiensNataliia PavliuchenkoJan KubovčiakTomáš BrdičkafalseTranscriptomic profiling of neutrophils from patients with chronic recurrent multifocal osteomyelitis (CRMO) and healthy age-matched controlsThe goal is to identify differntially expressed genes in multifocal osteomyelitis (CRMO) neutrophils to understand the mechanism of neutrophil dysregulation contributing to CRMO development.2026-09-18T00:00:00Z2026-09-18T14:14:00.663Z2026-02-10T09:51:44.31ZE-MTAB-16634EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184