{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Christopher Hoyle"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16640"],"description":["We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the response of these mice to Influenza A infection.  WT or mNLS mice were anaesthetized by 2.5% inhalation isoflurane and intranasally infected with 10^3 PFU of the IAV strain X31 (H3N2), in a volume of 30 µL PBS, on day 0. Infected WT and mNLS mice, and heterozygous control uninfected mice, were culled on day 7 post-IAV X31 infection, and the lung RNA was extracted."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.","Sample Collection - Infected WT, mNLS, and heterozygous control mice were culled on day 7 post-IAV X31 (H3N2) infection. One lung lobe was collected and placed in RNAlater stabilisation solution prior to subsequent RNA extraction.","Sequencing - Sequenced paired end 76bp on NOVASEQ6000 at the Genomic Technologies Core Facility University of Manchester","Nucleic Acid Extraction - Total RNA was extracted by transferring to a 2 mL tube containing RLT buffer supplemented with β-mercaptoethanol and a single 2 mm stainless steel bead. Tissue was homogenised into suspension using a Qiagen Tissue Lyser. Total RNA was then extracted using the RNeasy Mini Kit according to the manufacturer's protocol."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Christopher Hoyle"],"additional_accession":[]},"is_claimable":false,"name":"RNA-Seq of lung tissue from WT or interleukin-1alpha nuclear localisation sequence mutant mice infected with influenza A","description":"We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the response of these mice to Influenza A infection.  WT or mNLS mice were anaesthetized by 2.5% inhalation isoflurane and intranasally infected with 10^3 PFU of the IAV strain X31 (H3N2), in a volume of 30 µL PBS, on day 0. Infected WT and mNLS mice, and heterozygous control uninfected mice, were culled on day 7 post-IAV X31 infection, and the lung RNA was extracted.","dates":{"release":"2026-02-28T00:00:00Z","modification":"2026-02-28T02:02:24.619Z","creation":"2026-02-11T12:07:47.342Z"},"accession":"E-MTAB-16640","cross_references":{"ENA":["ERP188994"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0004184"]}}