biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChristopher HoyleIllumina NovaSeq 6000RNA-seq of total RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16640We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the response of these mice to Influenza A infection. WT or mNLS mice were anaesthetized by 2.5% inhalation isoflurane and intranasally infected with 10^3 PFU of the IAV strain X31 (H3N2), in a volume of 30 µL PBS, on day 0. Infected WT and mNLS mice, and heterozygous control uninfected mice, were culled on day 7 post-IAV X31 infection, and the lung RNA was extracted.biostudies-arrayexpressLibrary Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.Sample Collection - Infected WT, mNLS, and heterozygous control mice were culled on day 7 post-IAV X31 (H3N2) infection. One lung lobe was collected and placed in RNAlater stabilisation solution prior to subsequent RNA extraction.Sequencing - Sequenced paired end 76bp on NOVASEQ6000 at the Genomic Technologies Core Facility University of ManchesterNucleic Acid Extraction - Total RNA was extracted by transferring to a 2 mL tube containing RLT buffer supplemented with β-mercaptoethanol and a single 2 mm stainless steel bead. Tissue was homogenised into suspension using a Qiagen Tissue Lyser. Total RNA was then extracted using the RNeasy Mini Kit according to the manufacturer's protocol.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesChristopher HoylefalseRNA-Seq of lung tissue from WT or interleukin-1alpha nuclear localisation sequence mutant mice infected with influenza AWe generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the response of these mice to Influenza A infection. WT or mNLS mice were anaesthetized by 2.5% inhalation isoflurane and intranasally infected with 10^3 PFU of the IAV strain X31 (H3N2), in a volume of 30 µL PBS, on day 0. Infected WT and mNLS mice, and heterozygous control uninfected mice, were culled on day 7 post-IAV X31 infection, and the lung RNA was extracted.2026-02-28T00:00:00Z2026-02-28T02:02:24.619Z2026-02-11T12:07:47.342ZE-MTAB-16640ERP188994EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184