{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Christopher Hoyle"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16641"],"description":["We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the transcriptional response of primary BMDMs to LPS priming.  Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.","Sequencing - Sequenced paired end 59bp on NOVASEQ6000 at the Genomic Technologies Core Facility University of Manchester.","Nucleic Acid Extraction - Total RNA was extracted using a PureLink RNA mini kit.","Sample Collection - Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h). Supernatant was aspirated, cells washed once with PBS, and then lysis buffer containing 1% mercaptoethanol was added."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Christopher Hoyle"],"additional_accession":[]},"is_claimable":false,"name":"RNA-Seq of vehicle- vs LPS-treated primary BMDMs from WT or interleukin-1alpha nuclear localisation sequence mutant mice","description":"We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the transcriptional response of primary BMDMs to LPS priming.  Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h).","dates":{"release":"2026-02-28T00:00:00Z","modification":"2026-02-28T02:02:20.947Z","creation":"2026-02-11T12:11:00.129Z"},"accession":"E-MTAB-16641","cross_references":{"ENA":["ERP188995"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0005518","EFO_0004184"]}}