biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsChristopher HoyleIllumina NovaSeq 6000RNA-seq of total RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16641We generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the transcriptional response of primary BMDMs to LPS priming. Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h).biostudies-arrayexpressLibrary Construction - Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.Sequencing - Sequenced paired end 59bp on NOVASEQ6000 at the Genomic Technologies Core Facility University of Manchester.Nucleic Acid Extraction - Total RNA was extracted using a PureLink RNA mini kit.Sample Collection - Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h). Supernatant was aspirated, cells washed once with PBS, and then lysis buffer containing 1% mercaptoethanol was added.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesChristopher HoylefalseRNA-Seq of vehicle- vs LPS-treated primary BMDMs from WT or interleukin-1alpha nuclear localisation sequence mutant miceWe generated a mouse with a mutated IL-1alpha nuclear localisation sequence, causing loss of pro-IL-1alpha nuclear localisation. We tested whether this loss of nuclear pro-IL-1alpha affected the transcriptional response of primary BMDMs to LPS priming. Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h).2026-02-28T00:00:00Z2026-02-28T02:02:20.947Z2026-02-11T12:11:00.129ZE-MTAB-16641ERP188995EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184