{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["thomas cokelaer"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Myodes glareolus"],"species":["Myodes glareolus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16656"],"description":["Orthohantaviruses are rodent-borne viruses that can cause severe disease in humans but remain asymptomatic in their natural hosts. Increasing outbreaks and ecological changes raise important public health concerns. Understanding how these viruses interact with host cells is essential, particularly in reservoir species. Using RNA sequencing, the study compares cellular responses to different orthohantaviruses in bank vole kidney and respiratory cells. The results identify shared and virus- or tissue-specific regulatory factors linked to infection outcomes. Note that biological material originates from immortalized epithelial cell lines derived from bank vole (Myodes glareolus, NCBI Taxonomy ID: 447135). Mus musculus was selected in controlled vocabulary fields due to repository limitations and because mouse orthologs were used for functional annotation."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Libraries were constructed using a Illumina Stranded mRNA Prep (Illumina, USA) following the supplier's recommendations.","Nucleic Acid Extraction - bank vole cells were infected with orthohantaviruses at a MOI of 0.5 and RNA extracted 5 dpi. After washing with PBS, cells were treated with Trizol (Tri Reagent, Sigma Aldrich) and chloroform. The aqueous phase containing the RNA was precipitated with isopropanol and resuspended in distilled water.","Sequencing - RNA sequencing was performed on the Illumina NextSeq 500 platform.","Sample Collection - Bank vole cell lines (MyglaSWRecB, MyglaSWTrach, MyglaAEC; Evag catalogue) immortalized with SV40 T antigen and derived from kidney, tracheal, and alveolar epithelial tissues were obtained from Bonn University. Cells were cultured at 37 °C with 5% CO₂ in DMEM supplemented with 10% heat-inactivated fetal bovine serum. PUUV Sotkamo, PHV, and Tula Moravia virus strains were provided by collaborating laboratories in Berlin and Helsinki. Viral stocks were propagated in Vero E6 cells as previously described."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Myriam Ermonval","thomas cokelaer"],"additional_accession":[]},"is_claimable":false,"name":"Comparative transcriptomic responses of bank vole kidney and respiratory epithelial cells to distinct orthohantaviruses","description":"Orthohantaviruses are rodent-borne viruses that can cause severe disease in humans but remain asymptomatic in their natural hosts. Increasing outbreaks and ecological changes raise important public health concerns. Understanding how these viruses interact with host cells is essential, particularly in reservoir species. Using RNA sequencing, the study compares cellular responses to different orthohantaviruses in bank vole kidney and respiratory cells. The results identify shared and virus- or tissue-specific regulatory factors linked to infection outcomes. Note that biological material originates from immortalized epithelial cell lines derived from bank vole (Myodes glareolus, NCBI Taxonomy ID: 447135). Mus musculus was selected in controlled vocabulary fields due to repository limitations and because mouse orthologs were used for functional annotation.","dates":{"release":"2026-06-15T00:00:00Z","modification":"2026-06-15T01:01:17.442Z","creation":"2026-02-13T13:48:53.469Z"},"accession":"E-MTAB-16656","cross_references":{"ENA":["ERP189112"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}