biostudies-arrayexpressMarkus HeckmannMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16668This study comprises bulk RNA sequencing data generated from cardiac tissue of mice carrying a humanized MCL-1 receptor. Animals were treated with the selective MCL-1 inhibitor MIK665 or placebo. Cardiac tissue was collected at two experimental time points: during treatment (Day 14) and 7 days after last application (Day 21). The dataset was generated to characterize treatment- and time-dependent transcriptomic changes associated with pharmacological MCL-1 inhibition in vivo.biostudies-arrayexpressNucleic Acid Extraction - Frozen cardiac tissue was homogenized using a bead-based tissue lyser in RIPA buffer. Total RNA was isolated using TRIzol reagent followed by chloroform extraction and isopropanol precipitation according to standard protocols. RNA concentration was determined spectrophotometrically prior to library preparation.Library Construction - Poly(A)-containing mRNA was isolated using oligo(dT) beads and strand-specific mRNA libraries were generated according to standard BGI library preparation protocols, including RNA fragmentation, cDNA synthesis, adapter ligation, and PCR amplification.Sample Collection - Humanized Mcl-1 mice were euthanized at predefined experimental time points (Day 14 or Day 21). Hearts were rapidly excised at termination, rinsed briefly to remove residual blood, snap-frozen in liquid nitrogen, and stored at −80 °C until further processing. Blood sampling was performed at termination prior to tissue collection.Sequencing - Sequencing was performed on Illumina platforms using paired-end 150 bp reads (PE150) according to standard manufacturer protocols.Sequencing - Sequencing was performed on the DNBSEQ platform using paired-end 150 bp reads (PE150) according to standard manufacturer protocols.Library Construction - Poly(A)-containing mRNA was isolated using oligo(dT) beads and used for stranded mRNA library construction following standard Illumina-compatible protocols, including RNA fragmentation, cDNA synthesis, end repair, adapter ligation, and PCR amplification.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - Bulk RNA sequencing data were processed and analyzed in R (Version 4.5.2) using Bioconductor and CRAN packages. Paired-end FASTQ files were aligned to the mouse reference genome (GRCm39) using Rsubread (v2.24.0). Alignment was guided by a gene annotation file from GENCODE (version M37), and alignments were generated per sample using multithreaded processing. Gene-level quantification was performed from the resulting BAM files using Rsubread (v2.24.0). Reads overlapping annotated exons were summarized to gene-level counts. Mapping and assignment statistics were collected for each sample and consolidated across the dataset for quality control.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsDNBSEQ-G400Illumina HiSeq XRNA-seq of coding RNAMus musculusMarkus HeckmannfalseBulk RNA sequencing of cardiac tissue from MIK665-treated and placebo-treated humanized MCL-1 miceThis study comprises bulk RNA sequencing data generated from cardiac tissue of mice carrying a humanized MCL-1 receptor. Animals were treated with the selective MCL-1 inhibitor MIK665 or placebo. Cardiac tissue was collected at two experimental time points: during treatment (Day 14) and 7 days after last application (Day 21). The dataset was generated to characterize treatment- and time-dependent transcriptomic changes associated with pharmacological MCL-1 inhibition in vivo.2026-08-28T00:00:00Z2026-08-28T01:00:47.819Z2026-02-19T13:06:18.691ZE-MTAB-16668ERP189365EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003738EFO_0004184