{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Konstantinos Geles"],"organism":["Homo sapiens"],"software":["R v4.5.2","Affymetrix Transcrriptome Analysis Console software v.4.0"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16693"],"description":["Comparing AML cases with high vs. low CD81 surface protein levels. To gain mechanistic insights, we performed gene expression analysis on a subset of 45 diagnostic non CBF-AML samples. When comparing AML cases with high vs. low CD81 surface protein levels, we confirmed a strong association with CD81 mRNA expression, as expected."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total cellular RNA was extracted from a minimum of 5×10^6  cells with the use of Trizol reagent (GIBCO), and the RNA integrity was assessed by using an Agilent 2100 Bioanalyzer (Agilent).","Sample Collection - Bone marrow and peripheral blood were obtained before treatment, and mononucleated cells were isolated by means of sucrose density-gradient centrifugation (Ficoll-Paque) within 24 hours. Cells were re-suspended in RPMI 1640 medium (GIBCO) supplemented with 20 percent fetal-bovine serum (Integro).","Labeling - cDNA was synthesized using a T-7 linked oligo-dT primer, and cRNA was then synthesized with biotinylated UTP and CTP.","Scaning - Arrays were scanned using a laser confocal scanner (Affymetrix), and the expression value for each gene was calculated using Affymetrix Transcrriptome Analysis Console software v.4.0.","Hybridization - The labeled RNA was then fragmented and hybridized to HG_U133 Plus 2.0 oligonucleotide arrays (Affymetrix) according to the manufacturer's protocols."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Data Transformation - RMA normalization Method using affy v1.88.0 and limma v3.66 R v4.5.2, packages"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Agilent","PC"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["Meyling CHEOK","Konstantinos Geles"],"additional_accession":[]},"is_claimable":false,"name":"Microarray expression profiles of Acute Myeloid Leukemia patient samples","description":"Comparing AML cases with high vs. low CD81 surface protein levels. To gain mechanistic insights, we performed gene expression analysis on a subset of 45 diagnostic non CBF-AML samples. When comparing AML cases with high vs. low CD81 surface protein levels, we confirmed a strong association with CD81 mRNA expression, as expected.","dates":{"release":"2026-03-25T00:00:00Z","modification":"2026-03-25T02:03:18.719Z","creation":"2026-02-26T14:32:33.873Z"},"accession":"E-MTAB-16693","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003816","EFO_0003815"]}}