biostudies-arrayexpressKonstantinos GelesHomo sapiensR v4.5.2Affymetrix Transcrriptome Analysis Console software v.4.0https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16693Comparing AML cases with high vs. low CD81 surface protein levels. To gain mechanistic insights, we performed gene expression analysis on a subset of 45 diagnostic non CBF-AML samples. When comparing AML cases with high vs. low CD81 surface protein levels, we confirmed a strong association with CD81 mRNA expression, as expected.biostudies-arrayexpressNucleic Acid Extraction - Total cellular RNA was extracted from a minimum of 5×10^6 cells with the use of Trizol reagent (GIBCO), and the RNA integrity was assessed by using an Agilent 2100 Bioanalyzer (Agilent).Sample Collection - Bone marrow and peripheral blood were obtained before treatment, and mononucleated cells were isolated by means of sucrose density-gradient centrifugation (Ficoll-Paque) within 24 hours. Cells were re-suspended in RPMI 1640 medium (GIBCO) supplemented with 20 percent fetal-bovine serum (Integro).Labeling - cDNA was synthesized using a T-7 linked oligo-dT primer, and cRNA was then synthesized with biotinylated UTP and CTP.Scaning - Arrays were scanned using a laser confocal scanner (Affymetrix), and the expression value for each gene was calculated using Affymetrix Transcrriptome Analysis Console software v.4.0.Hybridization - The labeled RNA was then fragmented and hybridized to HG_U133 Plus 2.0 oligonucleotide arrays (Affymetrix) according to the manufacturer's protocols.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - RMA normalization Method using affy v1.88.0 and limma v3.66 R v4.5.2, packagesUnknownTranscriptomicsGenomicsProteomicsAgilentPCtranscription profiling by arrayHomo sapiensMeyling CHEOKKonstantinos GelesfalseMicroarray expression profiles of Acute Myeloid Leukemia patient samplesComparing AML cases with high vs. low CD81 surface protein levels. To gain mechanistic insights, we performed gene expression analysis on a subset of 45 diagnostic non CBF-AML samples. When comparing AML cases with high vs. low CD81 surface protein levels, we confirmed a strong association with CD81 mRNA expression, as expected.2026-03-25T00:00:00Z2026-03-25T02:03:18.719Z2026-02-26T14:32:33.873ZE-MTAB-16693EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815