biostudies-arrayexpressStephanie HannaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16698scRNAseq of thyroid eye disease orbital fat demonstrates fibroblast thyroid hormone signaling and SPARC production Orbital fat from four people undergoing decompression surgery for TED was digested to single cells and sequenced. Two donors' cells were hashed and combined into each sample. (i.e. four donors, two samples)biostudies-arrayexpressSample Collection - Samples of orbital fat were taken during surgery for planned or emergency orbital decompressionsNucleic Acid Extraction - To isolate single cells from the orbital fat, it was cut into small (2-3mm diameter) pieces and digested for 15 minutes in Hank’s buffered saline solution (HBSS) containing 2mg/ml collagenase P, 1mg/ml dispase and 1ul/ml DNaseI (100u/ml) at 37C with shaking. Samples were centrifuged and the fraction containing PBMCs and fibroblasts was frozen in CryostorCS10 (StemCell Technologies). Samples were then thawed, washed (BioLegend) and run on a 10xgenomics Single Cell 5’ Reagent kit v2.Sequencing - Samples were sequenced on an Illumina NextSeq, using a target read depth of 20,000 reads/cell for GEX and 5,000 reads/cells for cell hashing.Library Construction - Standard 10x V2 library construction. Cell hashing was insufficient but included for completenessOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - CellRanger 6.1 (10X Genomics), was used to align the reads obtained from sequencing refdata-gex-GRCh38-2020-A id name read pattern sequence feature_type D1 C0251H1 R2 5PNNNNNNNNNN(BC)NNNNNNNNN GTCAACTCTTTAGCG Antibody Capture D2 C0252H2 R2 5PNNNNNNNNNN(BC)NNNNNNNNN TGATGGCCTATTGGG Antibody Capture D3 C0252H3 R2 5PNNNNNNNNNN(BC)NNNNNNNNN TTCCGCCTCTCTTTG Antibody Capture D4 C0252H4 R2 5PNNNNNNNNNN(BC)NNNNNNNNN AGTAAGTTCAGCGTA Antibody CaptureMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNA from single cellsHomo sapiensStephanie HannafalsescRNAseq of cell from orbital fat in thyroid eye diseasescRNAseq of thyroid eye disease orbital fat demonstrates fibroblast thyroid hormone signaling and SPARC production Orbital fat from four people undergoing decompression surgery for TED was digested to single cells and sequenced. Two donors' cells were hashed and combined into each sample. (i.e. four donors, two samples)2026-06-26T00:00:00Z2026-06-26T01:00:44.246Z2026-03-02T14:04:53.872ZE-MTAB-16698ERP189782EFO_0002944EFO_0004170EFO_0005684EFO_0004917EFO_0005518EFO_0004184