biostudies-arrayexpressAyesa SyeninaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16701RNA was extracted from whole blood samples in Tempus tubes at baseline, day 1 (D1), D4, D6, and D28 after YF17D inoculation. We investigated differences in gene expression between obese (25<BMI<=35) and non-obese individuals (18<=BMI<=25) throughout the different timepoints.biostudies-arrayexpressSample Collection - Whole blood samples were collected in Tempus blood RNA tubes (Thermo Fisher Scientific, USA). Tempus tubes were stored at -80deg until RNA extraction.Sequencing - Then libraries with different indexs were multiplexed and loaded on an Illumina Novaseq instrument for sequencing using a 2x150 paired-end (PE) configuration according to manufacturer’s instructions.Nucleic Acid Extraction - RNA extraction from whole blood was performed using the Tempus Spin RNA Isolation kit (Thermo Scientific). RNA was eluted in 90ul of RNase-free water.Library Construction - 1 μg total RNA was used for following library preparation. The poly(A) mRNA isolation was performed using Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and high temperature. Priming was performed using Random Primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Pre-processing of data included removal of adapter sequences and filtering of low-quality reads (5' or 3' end bases that contains N’s or of quality values below 20) and contaminations (reads that are less than 75 bp long after trimming) by Cut adapt. Filtered data were subsequently aligned to reference genome using Hisat2 with default parameters. Differential gene expression analysis was subsequently conducted using DESeq2 package. Raw counts were imported into DESeq2 and genes with counts of less than 10 were filtered out prior to normalisation.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensAyesa SyeninafalseRNA-seq of peripheral whole blood samples comparing obese and non-obese individuals upon inoculation with yellow fever virus vaccine (YF17D)RNA was extracted from whole blood samples in Tempus tubes at baseline, day 1 (D1), D4, D6, and D28 after YF17D inoculation. We investigated differences in gene expression between obese (25<BMI<=35) and non-obese individuals (18<=BMI<=25) throughout the different timepoints.2026-03-14T00:00:00Z2026-03-14T02:03:46.982Z2026-02-27T19:07:42.786ZE-MTAB-16701ERP189734EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184