{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Wei Liu"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16722"],"description":["RNA sequencing was performed on heart tissue from genetically modified mice to evaluate the effects of gene modification and diabetic stress on RNA expression."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - paired end sequencing","Nucleic Acid Extraction - RNA was extracted using Trizol and treated with DNAse to eliminate genomic DNA contamination (Invitrogen) using the manufacturer's protocol.","Library Construction - Illumina stranded mRNA library","Sample Collection - Mice were subjected to HFHSD and STZ stress, and heart samples were snap frozen before RNA extraction"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Norm alisation was with DESeq2 v1.40.2","Sequence Alignment - The reads were mapped against the reference human genome (mm39) and counts per gene were calculated using annotation from GENCODE M35 (http://www.gencodegenes.org/) using STAR_2.7.7a (PMID: 23104886)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of total RNA"],"species":["Mus musculus"],"pubmed_authors":["Wei Liu"],"additional_accession":[]},"is_claimable":false,"name":"RNA sequencing on mouse heart tissue","description":"RNA sequencing was performed on heart tissue from genetically modified mice to evaluate the effects of gene modification and diabetic stress on RNA expression.","dates":{"release":"2026-08-14T00:00:00Z","modification":"2026-08-14T11:11:43.776Z","creation":"2026-03-05T11:08:26.182Z"},"accession":"E-MTAB-16722","cross_references":{"ENA":["ERP189933"],"Biostudies":["E-MTAB-16712"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}