biostudies-arrayexpressAgnieszka Witalisz-SieprackaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16774To assess the transcriptional differences between natural killer (NK) cells from patients with myeloproliferative neoplasms (MPN) carrying a mutation in calreticulin (CALR) and healthy donors (HD), bulk RNA sequencing was performed. Peripheral blood mononuclear cells (PBMC) were isolated from the blood of 4 HD and 4 CALR-mutant MPN patients using Ficoll density gradient and were cryopreserved after the isolation process. CD45+SSClowCD3-CD56+ cells were sorted from cryopreserved PBMC samples and RNA was isolated.biostudies-arrayexpressSample Collection - Patient and healthy donor NK cells (CD45+SSClowCD56+CD3-) were sorted from peripheral blood mononuclear cells (PBMCs) directly into RLT buffer (Quiagen) on a CytoflexSRT (Beckman Coulter).Library Construction - Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non strand specific library. Libraries were ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.Sequencing - Quantified libraries were pooled and sequenced on an Illumina platform.Nucleic Acid Extraction - RNA from sorted NK cells was purified using the RNeasy kit (Qiagen).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - FeatureCounts (2.0.6) was used to count the reads mapped to each gene.Sequence Alignment - Quality controlled reads were mapped to the human reference genome hg38 using HISAT2 (version 2.2.1).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensAgnieszka Witalisz-SieprackafalseRNA seq of natural killer (NK) cells of MPN patients with mutations in calreticulin (CALR) and healthy controlsTo assess the transcriptional differences between natural killer (NK) cells from patients with myeloproliferative neoplasms (MPN) carrying a mutation in calreticulin (CALR) and healthy donors (HD), bulk RNA sequencing was performed. Peripheral blood mononuclear cells (PBMC) were isolated from the blood of 4 HD and 4 CALR-mutant MPN patients using Ficoll density gradient and were cryopreserved after the isolation process. CD45+SSClowCD3-CD56+ cells were sorted from cryopreserved PBMC samples and RNA was isolated.2026-09-30T00:00:00Z2026-09-30T01:00:38.581Z2026-03-18T15:54:06.586ZE-MTAB-16774ERP190968EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184