biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsData Submission IFOM - ETSGridIONRNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16775We performed Nanopore direct sequencing in MEF R26 EYFP cells on a GridION device to detect EYFP RNA levels after DSB inductionbiostudies-arrayexpressLibrary Construction - RNA from each sample was depleted of the ribosomal RNA using the Illumina TruSeq Stranded Total RNA Library Prep Gold and used for library preparation. The complementary DNA strand was synthesized using an oligo dT adapter supplied by the kit. Then, adaptor-motor protein complexes were ligated to cDNA ends to allow cDNA to enter the pores for sequencing.Sequencing - Libraries were loaded on R9 flow cells using the Flow Cell Priming Kit (EXP-FLP002) and sequenced individually on GridION 5X device. Only one strand of the duplex is sequenced, producing 1D reads.Sample Collection - Cells were collected and washed with PBS 1x and RNA was extractedNucleic Acid Extraction - The cytoplasmic RNA fraction from MEF R26 EYFP cells, transfected with either E-C9 or sg-C9, was extracted using Maxwell RSC simplyRNA Tissue Kit (Promega).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Submission IFOM - ETSSara TavellaFabrizio D'Adda Di FagagnafalseDetecting EYFP RNA transcripts by Oxford Nanopore Technology in MEF R26 EYFPWe performed Nanopore direct sequencing in MEF R26 EYFP cells on a GridION device to detect EYFP RNA levels after DSB induction2026-05-15T00:00:00Z2026-05-15T01:00:41.436Z2026-03-18T16:00:01.71ZE-MTAB-16775ERP190969E-MTAB-16605EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184