biostudies-arrayexpressGeorge KassiotisHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16802H9 human embryonic stem cells (ESCs cells) were modified by CRISPR/Cas9 in order to inactivate the CALB1 gene. Wild-type (wt) and targeted (em) clones were multiplexed and sequenced as single cells.biostudies-arrayexpressSample Collection - All four cell populations, were grown in vitro and were processed as one batch at the same time.Sequencing - Paired-end libraries were sequenced on an Illumina NovaSeq X sequencer.Nucleic Acid Extraction - RNA was isolated and reverse transcribed with the 10× 3’-mRNA Seq (v3) kit (10× Genomics).Library Construction - Library prep was performed using the the 10× 3’-mRNA Seq (v3) kit (10× Genomics).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Data library-size normalization and Z-score scaling was performed using the 10x Genomics Cell Ranger Internal Algorithm.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNA from single cellsHomo sapiensGeorge KassiotisfalseSingle-cell RNA-Seq from CALB1wt and CALB1em H9 ESC clonesH9 human embryonic stem cells (ESCs cells) were modified by CRISPR/Cas9 in order to inactivate the CALB1 gene. Wild-type (wt) and targeted (em) clones were multiplexed and sequenced as single cells.2026-09-04T00:00:00Z2026-09-04T08:34:14.319Z2026-03-25T14:45:33.905ZE-MTAB-16802ERP191309E-MTAB-15900EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184