{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Kashyap Chhatbar"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16803"],"description":["Setd5 is a component of the mammalian SET3C complex, along with other components which are mutated in neuro-developmental disorders. We have generated SETD5 W834C heterozygous mice and have sequenced brain tissue for RNA-seq alongside wild-type littermate controls to identify differentially expressed genes and dysregulated pathways."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - DNA was removed using the TURBO DNAfree kit (Ambion) and RNA integrity was assessed on a bioanalyzer. 4µg RNA were used and ERCC Spike-ins were added to prepare sequencing libraries using the ScriptSeq Complete Gold Kit (Illumina). ScriptSeq Index Primers (Illumina) were used to multiplex libraries.","Nucleic Acid Extraction - Total RNA was isolated from mouse frontal cortex using Qiazol lysis reagent followed by purification with the RNeasy Mini kit (Qiagen) according to manufacturer protocol.","Sequencing - Samples were sequenced on an Illumina sequencer at Novogene.","Sample Collection - The frontal cortex was sectioned from the mice brain."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Differential gene expression analysis was performed using DESeq2.","Sequence Alignment - Annotation data was downloaded from Gencode and STAR aligner and Bowtie2 was used to align reads to the transcriptome and genome respectively."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of total RNA"],"species":["Mus musculus"],"pubmed_authors":["Jenna Hare","Kashyap Chhatbar","Jacky Guy","Adrian Bird","Katie Paton","Matthew Lyst","Tricia Mathieson"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of SETD5 W834C heterozygous mouse brains with matched controls","description":"Setd5 is a component of the mammalian SET3C complex, along with other components which are mutated in neuro-developmental disorders. We have generated SETD5 W834C heterozygous mice and have sequenced brain tissue for RNA-seq alongside wild-type littermate controls to identify differentially expressed genes and dysregulated pathways.","dates":{"release":"2026-04-27T00:00:00Z","modification":"2026-04-27T11:44:20.789Z","creation":"2026-03-25T14:37:06.125Z"},"accession":"E-MTAB-16803","cross_references":{"ENA":["ERP191308"],"Biostudies":["E-MTAB-15318"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0004184"]}}