biostudies-arrayexpressKashyap ChhatbarMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16803Setd5 is a component of the mammalian SET3C complex, along with other components which are mutated in neuro-developmental disorders. We have generated SETD5 W834C heterozygous mice and have sequenced brain tissue for RNA-seq alongside wild-type littermate controls to identify differentially expressed genes and dysregulated pathways.biostudies-arrayexpressLibrary Construction - DNA was removed using the TURBO DNAfree kit (Ambion) and RNA integrity was assessed on a bioanalyzer. 4µg RNA were used and ERCC Spike-ins were added to prepare sequencing libraries using the ScriptSeq Complete Gold Kit (Illumina). ScriptSeq Index Primers (Illumina) were used to multiplex libraries.Nucleic Acid Extraction - Total RNA was isolated from mouse frontal cortex using Qiazol lysis reagent followed by purification with the RNeasy Mini kit (Qiagen) according to manufacturer protocol.Sequencing - Samples were sequenced on an Illumina sequencer at Novogene.Sample Collection - The frontal cortex was sectioned from the mice brain.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Differential gene expression analysis was performed using DESeq2.Sequence Alignment - Annotation data was downloaded from Gencode and STAR aligner and Bowtie2 was used to align reads to the transcriptome and genome respectively.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of total RNAMus musculusJenna HareKashyap ChhatbarJacky GuyAdrian BirdKatie PatonMatthew LystTricia MathiesonfalseRNA-seq of SETD5 W834C heterozygous mouse brains with matched controlsSetd5 is a component of the mammalian SET3C complex, along with other components which are mutated in neuro-developmental disorders. We have generated SETD5 W834C heterozygous mice and have sequenced brain tissue for RNA-seq alongside wild-type littermate controls to identify differentially expressed genes and dysregulated pathways.2026-04-27T00:00:00Z2026-04-27T11:44:20.789Z2026-03-25T14:37:06.125ZE-MTAB-16803ERP191308E-MTAB-15318EFO_0002944EFO_0004170EFO_0009653EFO_0004917EFO_0005518EFO_0003816EFO_0004184