biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMartin SimonIllumina NovaSeq 6000-RNA-seq of total RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16823HepG2 cells were transfected with Gapmers against the NEAT1 ncRNA. THe experimental design inlcudes a negative control by scrabled Gapmers, and combination of Gapmer no.1 and no.3. Total RNA was sequenced after Ribo Depletion.biostudies-arrayexpressSample Collection - Gapmers were applied for NEAT1 Knokout, application of scambled GAPmers serves as a negative controlLibrary Construction - Ribosomal RNA was depleted and Illumina comaptible libraries generatedSequencing - Samles were sequenced on a Nova Seq 6000S4 laneNucleic Acid Extraction - RNA was isolated with Trizol and DNAse digestedOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMartin SimonfalseKnockdown of NEAT1 in HepG2 CellsHepG2 cells were transfected with Gapmers against the NEAT1 ncRNA. THe experimental design inlcudes a negative control by scrabled Gapmers, and combination of Gapmer no.1 and no.3. Total RNA was sequenced after Ribo Depletion.2026-04-15T00:00:00Z2026-04-17T01:01:31.611Z2026-03-30T12:15:12.522ZE-MTAB-16823ERP191548EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184