biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMartin SimonIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16825This study investigates the correlation of lncRNA Neat1 to liver inflammation and fibrosis. Different diets were applied to wt and Neat1 Knockout Mice: 1. normal diet 2. MCS: methionine-choline-supplemented diet 3. MCD: methionine-choline-deficiend diet The samples represent RNA Seq from liver.biostudies-arrayexpressNucleic Acid Extraction - To isolate total RNA from the mouse liver tissues, 200 µl of TRI Reagent® (Zymo Research Europe GmbH, Freiburg, Germany, catalogue no.: R2053) was dispensed into a 1.5 ml reaction tube. The liver tissues stored at -80°C were then placed on dry ice for the following procedure. A piece weighing approximately 5 mg was cut off with a scalpel and added to the TRI Reagent®. This was homogenised using the Mixy Professional tissue homogeniser (FastGene® Nippon Genetics, Düren, Germany), a further 200 µl of TRI Reagent® was added, the mixture was vortexed for 10 seconds and centrifuged for 1 minute at room temperature at 14.0 x g. The supernatant was transferred to a fresh 1.5 ml reaction tube and the RNA was then isolated using the kit in accordance with the manufacturer’s instructions. The elution volume was 50 µl.Sequencing - Libraries were dual indexed and sequenced on a Illumina Next Seq 6000S4 laneSample Collection - Following the three-week feeding period, the mice were euthanised by CO₂ followed by cardiac incision for serum preparation. The livers were excised, weighed and washed in PBS. The liver lobes were divided and each was flash-frozen in liquid nitrogen in a 1.5 ml reaction tube and stored at -80°C.Library Construction - Ribisomal RNA was depeleted using the NEB rRNA depeltion Kit and libraries for Illumina were preared using teh NEB NExt Ultra Express RNA Library Kit with 100ng input RNAOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMartin SimonfalseRNASeq of mice livers with different genotypesThis study investigates the correlation of lncRNA Neat1 to liver inflammation and fibrosis. Different diets were applied to wt and Neat1 Knockout Mice: 1. normal diet 2. MCS: methionine-choline-supplemented diet 3. MCD: methionine-choline-deficiend diet The samples represent RNA Seq from liver.2026-04-07T00:00:00Z2026-04-07T17:03:06.634Z2026-03-30T13:11:05.812ZE-MTAB-16825ERP191552EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184