{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Shad Hassan"],"organism":["Rattus norvegicus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16870"],"description":["We investigated neural populations mediating the effects of cagrilintide on energy balance in the dorsal vagal complex (DVC). Single-nucleus RNA sequencing and spatial transcriptomics were performed across mouse, rat, and macaque brainstem tissue. Treatment groups include diet-induced obese animals treated acutely or subchronically with cagrilintide or vehicle. The overarching aim was to identify and characterize the neuronal population showing the strongest and most sustained transcriptional response to treatment. Included in this ArrayExpress ID is the bulk-seq data for mouse and rat"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Sequencing was performed on a NovaSeq 6000 with a 2x150bp configuration, targeting approximately 20 million paired-end reads per sample","Nucleic Acid Extraction - Standard nucleic acid extraction was performed by Azenta","Sample Collection - For all experiments that involve RNA sequencing, brains were extracted after sacrifice and the hindbrain portion containing the DVC was dissected out. Fresh frozen tissues were preserved in RNAlater and stored on dry ice.","Library Construction - Standard nucleic acid library construction was performed by Azenta"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Mouse and rat samples were subjected to vsd-transformation using DESeq2 v1.30.1","Sequence Alignment - STAR v2.7.a3 was used for alignment and feature counting. The GRCm38 GENCODE vM23 genome and the genome were used for alignment of the mouse and rat data, respectively."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Rattus norvegicus"],"pubmed_authors":["Tune Pers","Shad Hassan"],"additional_accession":[]},"is_claimable":false,"name":"A Cross-Species Atlas of the Dorsal Vagal Complex Reveals Neural Mediators of Cagrilintide’s Effects on Energy Balance","description":"We investigated neural populations mediating the effects of cagrilintide on energy balance in the dorsal vagal complex (DVC). Single-nucleus RNA sequencing and spatial transcriptomics were performed across mouse, rat, and macaque brainstem tissue. Treatment groups include diet-induced obese animals treated acutely or subchronically with cagrilintide or vehicle. The overarching aim was to identify and characterize the neuronal population showing the strongest and most sustained transcriptional response to treatment. Included in this ArrayExpress ID is the bulk-seq data for mouse and rat","dates":{"release":"2026-06-08T00:00:00Z","modification":"2026-06-08T12:50:43.666Z","creation":"2026-04-08T10:00:03.424Z"},"accession":"E-MTAB-16870","cross_references":{"ENA":["ERP191836"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}