biostudies-arrayexpressFENG YANGCandida aurishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16883C. auris strain B12037 was exposed to tunicamycin. Transcriptomes of three mutants, TJ101 (#10), TJ107 (#16) and TJ110 (#19) were compared to parent. Cells were grown in YPD broth from OD600=0.1 till 1.0, then cells pellets were harvested.biostudies-arrayexpressSample Collection - Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen.Nucleic Acid Extraction - Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s recommendations. RNA degradation and contamination were monitored on 1% agarose gels. RNA purity was measured using a NanoPhotometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA). The RNA concentration was measured using the Qubit® RNA Assay Kit was used in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). RNA integrity was assessed with the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).Library Construction - Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA), following the manufacturer’s protocol. Index codes were added to attribute sequences to each sample. mRNA was purified from the total RNA with poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under an elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). A random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-) were used to synthesize the first strand cDNA. Then, second strand cDNA synthesis was performed using DNA Polymerase I and RNase H. Exonuclease/polymerase activities were used to convert the remaining overhangs into blunt ends. After adenylation of the 3’ ends of the DNA fragments, the NEBNext Adaptor with hairpin loop structures was ligated to prepare for hybridization. To select cDNA fragments of 150–200 bp in length, the AMPure XP system (Beckman Coulter, Beverly, USA) was used. 3 μl of the USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min, followed by a 5 min incubation at 95°C before PCR. PCR was then performed with the Phusion High-Fidelity DNA polymerase, Universal PCR primers and the Index (X) Primer. Finally, the PCR products were purified (AMPure XP System), and the library quality was assessed using the Agilent Bioanalyzer 2100 system. Clustering of the index-coded samples was performed using the cBot Cluster Generation System and the TruSeq PE Cluster Kit v3-cBot-HS (Illumina), according to the manufacturer’s instructions.Sequencing - After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2000 platform, and 125 bp paired-end reads were generated.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - fastp was used to trim adapter sequences and do quality trimming. Reads containing N >10% (N represents an unresolvable base) were defined as low quality and were discarded. Bases at the 3′-ends with quality lower than 20 were trimmed. If the remaining reads had quality lower than 10, the reads were also removed. If the reads after adapter trimming and quality trimming had length less than 20 bp, the reads were removed.UnknownTranscriptomicsGenomicsProteomicsDNBSEQ-T7RNA-seq of coding RNACandida aurisFENG YANGfalseComparative transcriptome analysis of tunicamycin selected mutants vs parentC. auris strain B12037 was exposed to tunicamycin. Transcriptomes of three mutants, TJ101 (#10), TJ107 (#16) and TJ110 (#19) were compared to parent. Cells were grown in YPD broth from OD600=0.1 till 1.0, then cells pellets were harvested.2026-04-25T00:00:00Z2026-04-25T01:02:31.784Z2026-04-10T13:03:55.869ZE-MTAB-16883ERP191985EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184