biostudies-arrayexpressAndrea MiccoliMugil cephalushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16923This experiments aimed at elucidating the hepatic transcriptional responses of the flathead grey mullet Mugil cephalus fed with a diet where conventional fishmeal was increasingly replaced with partially defatted black soldier fly (Hermetia illucens, BSF). This fish species is receiving increasing global attention in the aquaculture industry due to its favourable biological characteristics, including low trophic feeding habits and high adaptability to diverse environmental conditions, as well as its relatively low dietary protein requirements, making it a good candidate for production diversification.biostudies-arrayexpressSequencing - Illumina NovaSeq X Plus platform with the 150SE strategyLibrary Construction - Lexogene QuantSeq 3′ mRNA protocolSample Collection - Liver tissues from 9 replicates per dietary group were pooled into 3 experimental replicates of approximately 45 mg in weight to increase yield, consistency and representativity of the transcriptional signatures.Nucleic Acid Extraction - RNeasy Mini Kit (Qiagen, Hilden, Germany)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSequence Alignment - The raw reads were mapped to the M. cephalus reference genome (NCBI id GCF_022458985.1) using Dragen (version 3.9.5). Transcript quantification was performed using Dragen (version 3.9.5) and the GCF_022458985.1_CIBA_Mcephalus_1.1_genomic_utrs.gff annotationMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAMugil cephalusAndrea MiccolifalseRNA-seq of grey mullet Mugil cephalus liver in response to three increasing replacement levels (10%, 15% and 20%) of conventional fishmeal with black soldier fly Hermetia illucens mealThis experiments aimed at elucidating the hepatic transcriptional responses of the flathead grey mullet Mugil cephalus fed with a diet where conventional fishmeal was increasingly replaced with partially defatted black soldier fly (Hermetia illucens, BSF). This fish species is receiving increasing global attention in the aquaculture industry due to its favourable biological characteristics, including low trophic feeding habits and high adaptability to diverse environmental conditions, as well as its relatively low dietary protein requirements, making it a good candidate for production diversification.2026-07-27T00:00:00Z2026-07-27T06:29:17.262Z2026-04-17T09:26:02.123ZE-MTAB-16923ERP192286EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003738EFO_0004184