biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsJames GriffithsNextSeq 2000RNA-seq of coding RNA from single cellsMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16924Single-cell RNA sequencing was performed on murine oral tissue to characterise immune responses during Candida albicans infection. Control and IL-1RAcP knockout mice were analysed across a time course comprising naïve (uninfected), day 1, and day 2 post-infection samples. The dataset captures genotype-dependent differences in epithelial and immune cell populations and their transcriptional responses to infection. This study enables analysis of cell-type composition, activation states, and gene expression dynamics across infection and between genotypes.biostudies-arrayexpressSequencing - Libraries were sequenced on an Illumina platform according to standard 10x Genomics recommendations, generating paired-end reads (Read 1: cell barcode and UMI; Read 2: transcript sequence) along with index reads.Library Construction - Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium platform (Single Cell 3′ Gene Expression, v3/v3.1 chemistry) according to the manufacturer’s instructions. Briefly, single cells were encapsulated into droplets with barcoded gel beads to generate Gel Bead-in-Emulsions (GEMs), followed by reverse transcription, cDNA amplification, and library construction.Sample Collection - Mice were infected with Candida albicans as described in the main manuscript. At the indicated timepoints (naive, day 1, day 2 post-infection), tissues (tongue) were harvested, mechanically and enzymatically dissociated into single-cell suspensions, and filtered through a 40 µm cell strainer. Cell viability was assessed prior to downstream processing.Nucleic Acid Extraction - Single-cell suspensions were processed directly for library preparation. Cells were counted and viability confirmed (>85%) prior to loading onto the Chromium platform.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesJames GriffithsfalsescRNA-seq of control and IL-1RAcP knockout across Candida infection timecourseSingle-cell RNA sequencing was performed on murine oral tissue to characterise immune responses during Candida albicans infection. Control and IL-1RAcP knockout mice were analysed across a time course comprising naïve (uninfected), day 1, and day 2 post-infection samples. The dataset captures genotype-dependent differences in epithelial and immune cell populations and their transcriptional responses to infection. This study enables analysis of cell-type composition, activation states, and gene expression dynamics across infection and between genotypes.2026-06-16T00:00:00Z2026-06-16T01:00:36.908Z2026-04-17T09:27:50.898ZE-MTAB-16924ERP192288EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184