{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["JENIFFER MENDES DA SILVA FREIRE"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16930"],"description":["Human neutrophils isolated from peripheral blood of five healthy male donors were analyzed by one-color microarray to investigate transcriptional changes induced by single and sequential stimulation with platelet-activating factor (PAF) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). The experiment included five conditions: unstimulated control, PAF, fMLP, PAF followed by fMLP, and fMLP followed by PAF, totaling 25 samples. This design was used to evaluate whether the order of exposure to endogenous and exogenous inflammatory stimuli influences neutrophil transcriptional responses. Total RNA was extracted after stimulation and hybridized to the Clariom S Human Array according to the manufacturer’s protocol. The study aimed to characterize stimulus-dependent and order-dependent transcriptional profiles in human neutrophils."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Not applicable. Primary human neutrophils were freshly isolated from peripheral blood and were not cultured as a proliferating cell line.","Sample Treatment - Isolated neutrophils were distributed into five experimental conditions: unstimulated control, PAF stimulation, fMLP stimulation, PAF followed by fMLP, and fMLP followed by PAF. These conditions were designed to evaluate transcriptional responses to single and sequential inflammatory stimulation.","Nucleic Acid Extraction - Total RNA was extracted from isolated neutrophils after stimulation using the appropriate extraction protocol according to the manufacturer’s instructions. RNA quantity and purity were evaluated prior to downstream processing, and only samples with adequate quality were used for microarray analysis.","Labeling - RNA samples were processed and labeled according to the manufacturer’s protocol for the Affymetrix Clariom S Human Array. Briefly, total RNA was reverse transcribed, amplified, converted into biotin-labeled sense strand DNA targets, fragmented, and prepared for array hybridization","Hybridization - Biotin-labeled sense strand DNA targets were hybridized to the Affymetrix Clariom S Human Array according to the manufacturer’s instructions. After hybridization, arrays were washed and stained using the standard Affymetrix workflow.","Sample Collection - Human peripheral blood was collected from five healthy adult male donors after informed consent. Donors were screened according to the study inclusion and exclusion criteria. Neutrophils were isolated from fresh peripheral blood immediately after collection.","Scaning - Hybridized arrays were scanned using an Affymetrix GeneChip scanner according to the manufacturer’s instructions. Raw intensity data were generated with the Affymetrix software platform and exported as CEL files for downstream analysis."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["WAGNER FONTES","JENIFFER MENDES DA SILVA FREIRE"],"data_protocol":["Data Transformation - Raw microarray data were processed using Robust Multi-array Average (RMA), including background correction, quantile normalization, and probe set summarization. Normalized expression values were used for downstream analysis. Batch or donor-related effects were evaluated and corrected when appropriate."],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of human neutrophils after single and sequential stimulation with PAF and fMLP","description":"Human neutrophils isolated from peripheral blood of five healthy male donors were analyzed by one-color microarray to investigate transcriptional changes induced by single and sequential stimulation with platelet-activating factor (PAF) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). The experiment included five conditions: unstimulated control, PAF, fMLP, PAF followed by fMLP, and fMLP followed by PAF, totaling 25 samples. This design was used to evaluate whether the order of exposure to endogenous and exogenous inflammatory stimuli influences neutrophil transcriptional responses. Total RNA was extracted after stimulation and hybridized to the Clariom S Human Array according to the manufacturer’s protocol. The study aimed to characterize stimulus-dependent and order-dependent transcriptional profiles in human neutrophils.","dates":{"release":"2026-04-19T00:00:00Z","modification":"2026-04-19T20:06:23.65Z","creation":"2026-04-17T11:46:20.844Z"},"accession":"E-MTAB-16930","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003815","EFO_0003969"]}}