biostudies-arrayexpressDr. Satish MishraPlasmodium bergheihttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16953we perform RNA sequencing to characterize host and parasite transcriptomic changes in hepatocytes infected with Plasmodium berghei GAPs lacking either Stearoyl-CoA Desaturase (Scd) or Sporozoite Conserved Orthologous Transcript 1 (Scot1). Both knockouts elicit distinct host transcriptional responses, prominently affecting genes involved in cytoskeletal organization, cell adhesion, signaling, and lysosomal function. Scot1-deficient parasites exhibit reduced or no expression of Scd, indicating a possible regulatory link.biostudies-arrayexpressSequencing - libraries were paired-end sequenced on an Illumina 4000 sequencerSample Collection - HepG2 cells were seeded over night and infected with Genetically modified parasites to assess the exoerythrocytic phases.Library Construction - RNA sequencing libraries were prepared with an Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit.Nucleic Acid Extraction - Qiagen RNeasy micro kit and treated with DNase I in the column as per manufacturer’s instructionsMIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesData Transformation - minimum counts per million (CPM) threshold of 0.5. Data normalization and transformation were performed using the EdgeR package, applying a log2(CPM + C)MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of total RNAPlasmodium bergheiDr. Satish MishrafalseTranscriptomic dissection of host-parasite interactions reveals metabolic rewiring in hepatocytes infected with Plasmodium berghei GAPswe perform RNA sequencing to characterize host and parasite transcriptomic changes in hepatocytes infected with Plasmodium berghei GAPs lacking either Stearoyl-CoA Desaturase (Scd) or Sporozoite Conserved Orthologous Transcript 1 (Scot1). Both knockouts elicit distinct host transcriptional responses, prominently affecting genes involved in cytoskeletal organization, cell adhesion, signaling, and lysosomal function. Scot1-deficient parasites exhibit reduced or no expression of Scd, indicating a possible regulatory link.2026-05-15T00:00:00Z2026-05-15T01:00:42.045Z2026-04-23T11:35:38.101ZE-MTAB-16953EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184