biostudies-arrayexpressGiulio AcetoHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16956RNA-seq samples in SCCOHT-1 and COV434 for idasanutlin treatment (0.5μM, 24h), and RNA-seq samples in BIN-67 and COV434 for short-term idasanutlin treatment (0.5μM, 3h). The goal of the experiment is to assess the genes that are regulated by p53 stabilization in the long term or short term using idasanutlin treatment in SCCOHT.biostudies-arrayexpressSequencing - Samples were sequenced with an illumina Next Seq 500 instrumentLibrary Construction - 1ug total RNA was used for the library construction. RNA libraries for RNA-Seq were prepared using the SMARTER mRNA-Seq library.Sample Collection - BIN-67, COV434 and SCCOHT-1 cells were cultured in Roswell Park Memorial Institute 1640 Medium (RPMI, Thermo Fisher Scientific, Cat# 11875-093; no pyruvate) with 6% fetal bovine serum (Sigma, Cat# F1051), 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat# 15140-122), and 2 mM L-glutamine (Thermo Fisher Scientific, Cat# 25030-081). Cells were maintained in a humidified 5% CO2-containing incubator at 37°C and regular Mycoplasma test was performed using Mycoalert Detection Kit (Lonza, Cat# LT07-318). Cells were treated with 0.5μM A-485 for 24h or 3h. Total RNA was collected with the RNeasy Mini Kit (Qiagen, Hilden, Germany).Nucleic Acid Extraction - Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Sequencing files were aligned to the human reference genome (hg38) using STAR (v2.6.1c). Gene expression levels were quantified using Homer or HTseq with Gencode gene annotation (GTF) files. Differentially expressed genes were identified using Edger.UnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNAHomo sapiensGiulio AcetoSidong HuangfalseLong term and short term Idasanutlin treatments in SCCOHT cell linesRNA-seq samples in SCCOHT-1 and COV434 for idasanutlin treatment (0.5μM, 24h), and RNA-seq samples in BIN-67 and COV434 for short-term idasanutlin treatment (0.5μM, 3h). The goal of the experiment is to assess the genes that are regulated by p53 stabilization in the long term or short term using idasanutlin treatment in SCCOHT.2026-05-05T00:00:00Z2026-05-05T17:57:54.702Z2026-04-23T11:59:16.822ZE-MTAB-16956ERP192500EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184