{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["JENIFFER MENDES DA SILVA FREIRE"],"study_type":["microRNA profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16957"],"description":["This study evaluated the miRNA expression profile of human neutrophils under resting conditions and after stimulation with PAF and fMLP, applied alone or sequentially. The objective was to determine whether distinct inflammatory stimuli and their order of exposure induce different miRNA expression patterns in neutrophils. Neutrophils from healthy donors were assigned to five conditions: control, PAF, fMLP, PAF followed by fMLP (PF), and fMLP followed by PAF (FP). Total RNA was extracted and hybridized to the Affymetrix miRNA 4.0 Array platform for expression analysis."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was extracted from isolated human neutrophils after the experimental treatments, following the manufacturer's instructions for the extraction method used in the study. RNA quantity and quality were assessed prior to downstream analysis. The extracted RNA was used for miRNA expression profiling on the Affymetrix miRNA 4.0 Array platform.","Growth Protocol - Human neutrophils were freshly isolated from peripheral blood and were not cultured for long-term growth or expansion prior to treatment.","Scaning - Arrays were scanned using an Affymetrix scanner according to the manufacturer's instructions. Raw signal intensities were extracted with Affymetrix software and exported as CEL files for downstream analysis.","Labeling - RNA samples were labeled with biotin according to the standard protocol recommended by the manufacturer for the Affymetrix miRNA 4.0 Array platform.","Hybridization - Biotin-labeled RNA samples were hybridized to the Affymetrix miRNA 4.0 Array according to the manufacturer's instructions. After hybridization, the arrays were washed and stained using the standard Affymetrix workflow.","Sample Collection - Peripheral blood samples were obtained from five healthy adult male donors after informed consent and according to the inclusion and exclusion criteria established for the study. Donors were between 18 and 35 years old and had no active disease, recent infection, recent surgery, continuous medication use, smoking, recent alcohol intake, or recent strenuous physical exercise. Neutrophils were isolated from peripheral blood immediately after collection for downstream experimental procedures.","Sample Treatment - Freshly isolated human neutrophils were distributed into five experimental conditions: unstimulated control, PAF, fMLP, PAF followed by fMLP, and fMLP followed by PAF. Single-stimulus and sequential-stimulus conditions were used to evaluate the effects of inflammatory cues and the order of stimulation on miRNA expression in neutrophils."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["WAGNER FONTES","JENIFFER MENDES DA SILVA FREIRE"],"data_protocol":["Data Transformation - When applicable, donor-related effects were considered during downstream analysis."],"additional_accession":[]},"is_claimable":false,"name":"miRNA expression profiling of human neutrophils after single and sequential stimulation with PAF and fMLP","description":"This study evaluated the miRNA expression profile of human neutrophils under resting conditions and after stimulation with PAF and fMLP, applied alone or sequentially. The objective was to determine whether distinct inflammatory stimuli and their order of exposure induce different miRNA expression patterns in neutrophils. Neutrophils from healthy donors were assigned to five conditions: control, PAF, fMLP, PAF followed by fMLP (PF), and fMLP followed by PAF (FP). Total RNA was extracted and hybridized to the Affymetrix miRNA 4.0 Array platform for expression analysis.","dates":{"release":"2026-05-10T00:00:00Z","modification":"2026-05-10T01:02:03.912Z","creation":"2026-04-24T12:20:45.898Z"},"accession":"E-MTAB-16957","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0000753","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003815","EFO_0003969"]}}