{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Shengduo Pei"],"instrument_platform":["DNBSEQ-G400"],"study_type":["RNA-seq of coding RNA from single cells"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16990"],"description":["The B16F10 mouse melanoma cells were inoculated onto the right flank of C57BL/6J mice and every 4 days, anti-PD-1 antibody was injected through the tail vein. In total, 3 doses of immunotherapy treatments were given to the experimental animals. Two days after the last treatment, all tumors including treated and untreated control were collected, cut and digested. The resulted tumor sample single cell suspensions were next red blood cell lysed, stained and then proceeded for FACS sorting to get the live CD45+ intratumoral immune cells. The CD45+ cells from the tumor samples were subsequently count to adjust cell concentration and then used as input for scRNA-seq library construction using the 10x Genomics GEM-X Universal 3' Gene Expression v4 kit following the manufacturer’s protocol."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - According to the manufacturer’s 10x Genomics GEM-X Universal 3' Gene Expression v4 kit instructions.","Sample Collection - The B16F10 mouse melanoma cells were inoculated onto the right flank of C57BL/6J mice and every 4 days, anti-PD-1 antibody was injected through the tail vein. In total, 3 doses of immunotherapy treatments were given to the experimental animals. Two days after the last treatment, all tumors including treated and untreated control were collected, cut and digested. The resulted tumor sample single cell suspensions were next red blood cell lysed, stained and then proceeded for FACS sorting to get the live CD45+ intratumoral immune cells. The CD45+ cells from the tumor samples were subsequently count to adjust cell concentration and then used as input for scRNA-seq library construction using the 10x Genomics GEM-X Universal 3' Gene Expression v4 kit following the manufacturer’s protocol.","Nucleic Acid Extraction - According to the manufacturer’s 10x Genomics GEM-X Universal 3' Gene Expression v4 kit user instructions.","Library Construction - According to the manufacturer’s 10x Genomics GEM-X Universal 3' Gene Expression v4 kit user instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Shengduo Pei"],"additional_accession":[]},"is_claimable":false,"name":"scRNA-seq of CD45+ cells from mouse B16F10 melanoma treated with anti-PD-1 antibody against untreated control","description":"The B16F10 mouse melanoma cells were inoculated onto the right flank of C57BL/6J mice and every 4 days, anti-PD-1 antibody was injected through the tail vein. In total, 3 doses of immunotherapy treatments were given to the experimental animals. Two days after the last treatment, all tumors including treated and untreated control were collected, cut and digested. The resulted tumor sample single cell suspensions were next red blood cell lysed, stained and then proceeded for FACS sorting to get the live CD45+ intratumoral immune cells. The CD45+ cells from the tumor samples were subsequently count to adjust cell concentration and then used as input for scRNA-seq library construction using the 10x Genomics GEM-X Universal 3' Gene Expression v4 kit following the manufacturer’s protocol.","dates":{"release":"2026-05-16T00:00:00Z","modification":"2026-05-16T01:01:42.642Z","creation":"2026-05-01T11:38:36.199Z"},"accession":"E-MTAB-16990","cross_references":{"ENA":["ERP192923"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}