{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Yihang Shen"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["ChIP-seq"],"organism":["Rattus rattus"],"species":["Rattus rattus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16991"],"description":["Two rat TN models were established, including infraorbital nerve chronic constriction injury (IoN-CCI, TN-No-DeMy) and trigeminal root entry zone (REZ) micro-compression (TN-DeMy). Myelin regulatory factor (MYRF) over-expression (MOE) or knockdown (MKD), carbamazepine (CBZ), AP-1 inhibitor SR"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Schwann cells are washed by PBS twice, and resuspended by 1 mL RIPA buffer, and put on ice for 30 min, followed by highest speed centrifugation for 15 min. The supernant containing with nucleus are transfered to another new tube.","Sample Collection - Schwann tissues at REZ of different TN groups were harvested.","Sequencing - DNA library with ligated adapters were isolated based on appropriate size for sequencing using the Illumina NovaSeq6000 platform.","Library Construction - whole cell lysates are sonicated to break up the genomic DNA into 200-500 bp fragments. Then, 10% lysates were saved as input and the remaining were incubated with 1 μg IP grade antibody of SOX10 at 4oC overnight. This is followed by incubating for 2 h with Protein A beads (Thermo Fisher Scientific) at 37 °C to pull down the bound DNA fragments. DNA fragments from the pull-down is isolated by ethanol precipitation.For high throughput sequencing, we added 3'-dA overhang to the SOX10 enriched or input DNA and ligated them to the adapter to build a DNA library."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Yihang Shen"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide occupancy of SOX10 in Schwann cells of rat trigeminal neuralgia","description":"Two rat TN models were established, including infraorbital nerve chronic constriction injury (IoN-CCI, TN-No-DeMy) and trigeminal root entry zone (REZ) micro-compression (TN-DeMy). Myelin regulatory factor (MYRF) over-expression (MOE) or knockdown (MKD), carbamazepine (CBZ), AP-1 inhibitor SR","dates":{"release":"2026-05-22T00:00:00Z","modification":"2026-05-22T01:01:37.197Z","creation":"2026-05-01T11:26:26.982Z"},"accession":"E-MTAB-16991","cross_references":{"ENA":["ERP192921"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002692","EFO_0005518","EFO_0004184"]}}