biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsYihang ShenIllumina NovaSeq 6000ChIP-seqRattus rattusRattus rattushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16991Two rat TN models were established, including infraorbital nerve chronic constriction injury (IoN-CCI, TN-No-DeMy) and trigeminal root entry zone (REZ) micro-compression (TN-DeMy). Myelin regulatory factor (MYRF) over-expression (MOE) or knockdown (MKD), carbamazepine (CBZ), AP-1 inhibitor SRbiostudies-arrayexpressNucleic Acid Extraction - Schwann cells are washed by PBS twice, and resuspended by 1 mL RIPA buffer, and put on ice for 30 min, followed by highest speed centrifugation for 15 min. The supernant containing with nucleus are transfered to another new tube.Sample Collection - Schwann tissues at REZ of different TN groups were harvested.Sequencing - DNA library with ligated adapters were isolated based on appropriate size for sequencing using the Illumina NovaSeq6000 platform.Library Construction - whole cell lysates are sonicated to break up the genomic DNA into 200-500 bp fragments. Then, 10% lysates were saved as input and the remaining were incubated with 1 μg IP grade antibody of SOX10 at 4oC overnight. This is followed by incubating for 2 h with Protein A beads (Thermo Fisher Scientific) at 37 °C to pull down the bound DNA fragments. DNA fragments from the pull-down is isolated by ethanol precipitation.For high throughput sequencing, we added 3'-dA overhang to the SOX10 enriched or input DNA and ligated them to the adapter to build a DNA library.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYihang ShenfalseGenome-wide occupancy of SOX10 in Schwann cells of rat trigeminal neuralgiaTwo rat TN models were established, including infraorbital nerve chronic constriction injury (IoN-CCI, TN-No-DeMy) and trigeminal root entry zone (REZ) micro-compression (TN-DeMy). Myelin regulatory factor (MYRF) over-expression (MOE) or knockdown (MKD), carbamazepine (CBZ), AP-1 inhibitor SR2026-05-22T00:00:00Z2026-05-22T01:01:37.197Z2026-05-01T11:26:26.982ZE-MTAB-16991ERP192921EFO_0002944EFO_0004170EFO_0002692EFO_0005518EFO_0004184