biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsCathy SlackIllumina HiSeq 1500RNA-seq of coding RNADrosophila melanogasterDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17017The transcriptional response to the small molecule MEK inhbitor, trametinib, was analysed from RNA extracts prepared from 10-day old adult female Drosophila that had been maintained under normal and high sugar dietary conditions post-eclosion.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from whole adult female flies by homogenisation in TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. Following chloroform extraction, RNAs were purified using RNeasy Mini columns (Qiagen) including an on-column DNase digestion.Sequencing - RNA-seq libraries were sequenced on an Illumina HiSeq 1500 platform, paired-end read 150 bp, according to the manufacturer’s instructions.Library Construction - RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep kit (Illumina). Libraries were quantified on a Bioanalyzer (Agilent Technologies).Sample Treatment - Flies were maintained on two different diets: normal (1x) sugar or high (8x sugar) with either vehicle alone (DMSO) or 15.6uM trametinib (added from a stock solution of trametinib dissolved in DMSO). Final DMSO concentration was maintained at 0.025%.Sample Collection - Adult flies were reared at standard density on sugar-yeast-agar (SYA) media. Newly eclosed adults were transfered to fresh culture vessels and incubated at 25C for 24h before being sorted by sex. Female flies were transferred to vials (15 flies per vial) and incubated until 10 days of age when they were snap frozen in liquid nitrogen and stored at -80C.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesCathy SlackfalseRNA-seq of Drosophila exposed to trametinib under normal and high sugar dietary conditionsThe transcriptional response to the small molecule MEK inhbitor, trametinib, was analysed from RNA extracts prepared from 10-day old adult female Drosophila that had been maintained under normal and high sugar dietary conditions post-eclosion.2026-09-03T00:00:00Z2026-09-03T08:54:52.55Z2026-05-06T12:45:48.736ZE-MTAB-17017ERP193087EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184EFO_0003969