{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["David Toppe"],"organism":["Drosophila melanogaster"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17037"],"description":["This Dataset contains global mRNA expression data of 10 day old female adult Drosophila melanogaster heads in which autophagy, a fundamental cellular degradation pathway in contexts of health and disease, is impaired in a major brain region, the Mushroombody, by RNAi mediated knockdown of Atg5 (ok107-Gal4>UAS-Atg5-RNAi). The control group consists of an age- and sex-matched wildtypic Drosophila strain (ok107-Gal4/+).  4 biological replicates per genotype were processed, each sample containing around 50 Drosophila heads. Samples labelled with AB1-AB4 refer to the control group, samples labelled with XY1-XY4 to the Atg5 knockdown group."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Nucleic acid library construction was performed by Lexogen using the QuantSeq method.","Nucleic Acid Extraction - Nucleic acid extraction was performed by Lexogen using the QuantSeq method.","Sequencing - Nucleic acid sequencing was performed by Lexogen using the QuantSeq method.","Sample Collection - Adult Drosophila female flies, raised for 10 days at 29°C, were snap-frozen in liquid nitrogen and stored at -80 degrees until sample collection of all biological replicates was completed. Flies were subsequently decapitated mechanically and fly heads were again stored at -80 degrees. Samples were shipped on dry ice for further processing by Lexogen."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Reads were aligned to a reference genome with STAR aligner. The differential expression analysis was performed with the DeSeq2 bioconducter package by Lexogen"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina iSeq 100"],"study_type":["RNA-seq of coding RNA"],"species":["Drosophila melanogaster"],"pubmed_authors":["David Toppe"],"additional_accession":[]},"is_claimable":false,"name":"RNA Seq Data of adult Drosophila melanogaster heads with Mushroombody-specific knockdown of Atg5 in comparison to wildtype controls","description":"This Dataset contains global mRNA expression data of 10 day old female adult Drosophila melanogaster heads in which autophagy, a fundamental cellular degradation pathway in contexts of health and disease, is impaired in a major brain region, the Mushroombody, by RNAi mediated knockdown of Atg5 (ok107-Gal4>UAS-Atg5-RNAi). The control group consists of an age- and sex-matched wildtypic Drosophila strain (ok107-Gal4/+).  4 biological replicates per genotype were processed, each sample containing around 50 Drosophila heads. Samples labelled with AB1-AB4 refer to the control group, samples labelled with XY1-XY4 to the Atg5 knockdown group.","dates":{"release":"2026-06-30T00:00:00Z","modification":"2026-06-30T01:01:10.303Z","creation":"2026-05-14T12:23:00.364Z"},"accession":"E-MTAB-17037","cross_references":{"ENA":["ERP193432"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}