biostudies-arrayexpressDavid ToppeDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17037This Dataset contains global mRNA expression data of 10 day old female adult Drosophila melanogaster heads in which autophagy, a fundamental cellular degradation pathway in contexts of health and disease, is impaired in a major brain region, the Mushroombody, by RNAi mediated knockdown of Atg5 (ok107-Gal4>UAS-Atg5-RNAi). The control group consists of an age- and sex-matched wildtypic Drosophila strain (ok107-Gal4/+). 4 biological replicates per genotype were processed, each sample containing around 50 Drosophila heads. Samples labelled with AB1-AB4 refer to the control group, samples labelled with XY1-XY4 to the Atg5 knockdown group.biostudies-arrayexpressLibrary Construction - Nucleic acid library construction was performed by Lexogen using the QuantSeq method.Nucleic Acid Extraction - Nucleic acid extraction was performed by Lexogen using the QuantSeq method.Sequencing - Nucleic acid sequencing was performed by Lexogen using the QuantSeq method.Sample Collection - Adult Drosophila female flies, raised for 10 days at 29°C, were snap-frozen in liquid nitrogen and stored at -80 degrees until sample collection of all biological replicates was completed. Flies were subsequently decapitated mechanically and fly heads were again stored at -80 degrees. Samples were shipped on dry ice for further processing by Lexogen.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Reads were aligned to a reference genome with STAR aligner. The differential expression analysis was performed with the DeSeq2 bioconducter package by LexogenMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina iSeq 100RNA-seq of coding RNADrosophila melanogasterDavid ToppefalseRNA Seq Data of adult Drosophila melanogaster heads with Mushroombody-specific knockdown of Atg5 in comparison to wildtype controlsThis Dataset contains global mRNA expression data of 10 day old female adult Drosophila melanogaster heads in which autophagy, a fundamental cellular degradation pathway in contexts of health and disease, is impaired in a major brain region, the Mushroombody, by RNAi mediated knockdown of Atg5 (ok107-Gal4>UAS-Atg5-RNAi). The control group consists of an age- and sex-matched wildtypic Drosophila strain (ok107-Gal4/+). 4 biological replicates per genotype were processed, each sample containing around 50 Drosophila heads. Samples labelled with AB1-AB4 refer to the control group, samples labelled with XY1-XY4 to the Atg5 knockdown group.2026-06-30T00:00:00Z2026-06-30T01:01:10.303Z2026-05-14T12:23:00.364ZE-MTAB-17037ERP193432EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184