biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMark TurnerIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17054RNA-Sequencing of Human LHCN-M2 skeletal muscle myotubes exposed to 8 hours of Electrical Pulse Stimulation (12v, 2ms, 3ms) to establish the transcriptional changes to an exercise mimetic. LHCN-M2 human skeletal muscle myoblasts were differentiated into multinucleated myotubes before being exposed to EPS.biostudies-arrayexpressLibrary Construction - Human mRNA Sequencing (WOBI) conducted by Novogene. mRNA library preparation (poly A enrichment). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.Sequencing - NovaSeq X Plus Series (PE150) (6G raw data per sample)Nucleic Acid Extraction - RNA Extraction conducted using Monarch® Total RNA Miniprep Kit according to manufactures protocolsSample Collection - Samples were collected in RNA Lysis buffer from Monarch® Total RNA Miniprep Kit according to the manufacturer's guidelines.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMark TurnerfalseRNA-Seq of Human LHCN-M2 skeletal muscle myotubes to Electrical Pulse StimulationRNA-Sequencing of Human LHCN-M2 skeletal muscle myotubes exposed to 8 hours of Electrical Pulse Stimulation (12v, 2ms, 3ms) to establish the transcriptional changes to an exercise mimetic. LHCN-M2 human skeletal muscle myoblasts were differentiated into multinucleated myotubes before being exposed to EPS.2026-06-30T00:00:00Z2026-06-30T01:01:11.942Z2026-05-15T17:07:55.167ZE-MTAB-17054ERP193478EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184