biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMartin SimonIllumina HiSeq 2500RNA-seq of coding RNADrosophila melanogasterDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17059We adapted the yeast clogger system to Drosophila motoneurons to block TOM-TIM23-mediated import with temporal control. Sustained import stress converts somatic mitochondria into donut-shaped structures, depletes functional mitochondria from synaptic terminals, and causes progressive neurodegeneration with impaired neurotransmitter release and locomotor dysfunction. This neurodegeneration is mechanistically distinct from mitochondrial absence, as miro mutant neurons that completely lack presynaptic mitochondria do not degenerate. This dataset includes RNA-seq reads from an experiment when mito-IMMDHFR expression was induced pan-neuronally using the TARGET system (n-syb-Gal4; tub-Gal80ts) by shifting animals from 18°C to 29°C for 1, 2, or 3 days prior to collection at the wandering third instar larval stage.biostudies-arrayexpressSample Collection - For each condition, 15 brains from wandering third instar larvae were dissected and pooled per biological replicate from 3 independent crosses. Brains were transferred into 1.5 ml tubes containing 250 µl peqGOLD TriFast (peqlab) and homogenised.Sequencing - Sequencing was performed on a HiSeq 2500 (Illumina) with single-end 50 bp reads.Nucleic Acid Extraction - RNA was isolated according to manufacturer's instructions, digested with DNase with subsequent purification with acid phenol.Library Construction - After integrity check with the Bioanalyzer RNA Pico Chips (Agilent), Illumina-compatible RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMartin SimonfalseRNA-seq of induced mitochronrial import stress and neurodegeneration in DrosophilaWe adapted the yeast clogger system to Drosophila motoneurons to block TOM-TIM23-mediated import with temporal control. Sustained import stress converts somatic mitochondria into donut-shaped structures, depletes functional mitochondria from synaptic terminals, and causes progressive neurodegeneration with impaired neurotransmitter release and locomotor dysfunction. This neurodegeneration is mechanistically distinct from mitochondrial absence, as miro mutant neurons that completely lack presynaptic mitochondria do not degenerate. This dataset includes RNA-seq reads from an experiment when mito-IMMDHFR expression was induced pan-neuronally using the TARGET system (n-syb-Gal4; tub-Gal80ts) by shifting animals from 18°C to 29°C for 1, 2, or 3 days prior to collection at the wandering third instar larval stage.2026-06-18T00:00:00Z2026-06-18T01:00:36.391Z2026-05-19T15:17:49.675ZE-MTAB-17059ERP193603EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184