{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Carl MANN"],"organism":["Homo sapiens"],"software":["bowtie2, samtools, sambamba"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17100"],"description":["Determine the contribution of repressive H3-K27me3 marks on differential gene expression in primary human fibroblasts from fetal lung (WI38), adult lung, adult abdomen, and adult breast under conditions of proliferation, and for the WI38 cells, in senescence induced by etoposide."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - PCR amplification of Cut & Tag fragmented DNA using Illmina multiplex oligonucleotide primers.","Sequencing - Illumina NextSeq sequencing of multiplexed libraries","Sample Collection - Trypsinized cells and used 50,000 for Cut & Tag with the nuclear permeabilization protocol described by the Henikoff lab.","Nucleic Acid Extraction - Phenol-chloroform extraction after the Cut & Tag reaction followed by ethanol precipitation."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Fastq.gz files were aligned to the GRCh38 genome using bowtie2 with the following parameters: --end-to-end --very-sensitive --no-mixed --no-discordant -k 1 -X 1000 -I 25. The resulting sam files were converted to bam files with samtools view. Duplicates were removed with sambamba markdup -r and reads mapping to the mitochondrial genome were removed."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 550"],"study_type":["CUT&Tag"],"species":["Homo sapiens"],"pubmed_authors":["Carl MANN"],"additional_accession":[]},"is_claimable":false,"name":"H3-K27me3 Cut & Tag of primary human fibroblasts","description":"Determine the contribution of repressive H3-K27me3 marks on differential gene expression in primary human fibroblasts from fetal lung (WI38), adult lung, adult abdomen, and adult breast under conditions of proliferation, and for the WI38 cells, in senescence induced by etoposide.","dates":{"release":"2026-07-09T00:00:00Z","modification":"2026-07-09T17:24:59.28Z","creation":"2026-05-27T12:52:44.916Z"},"accession":"E-MTAB-17100","cross_references":{"ENA":["ERP194048"],"Biostudies":["E-MTAB-14625","E-MTAB-16030","E-MTAB-14201"],"EFO":["EFO_0002944","EFO_0920004","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0004184"]}}