biostudies-arrayexpressPaul RégnierHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17120Behçet's disease (BD) is a systemic vasculitis with inflammatory lesions mediated by cytotoxic T cells and neutrophils. Here, we explore the critical involvement of NF-κB signaling pathway in proinflammatory CD8+ T cells differentiation and disease progress of BD patients. Along with other experimental techniques, we performed microarray gene expression analyses of CD8+ T cells from BD patients and HD. Throughout the manuscript, we highlighted that activated CD8+ T cells through NF-κB signaling pathway are instrumental in BD.biostudies-arrayexpressHybridization - Afterwards, prepared samples were hybridized to Human HT-12 v4 Beadarrays.Scaning - Then, raw IDAT files were processed using illuminaio R package and concatenated into a single text file. Probe names were then converted to HGNC symbols using BioMart R package. Probes being annotated to the same gene were merged by mean.Nucleic Acid Extraction - Total RNA from CD8+ T cells was then extracted using the NucleoSpin RNA kit (Macherey-Nagel) and quantified by NanoDrop ND-1000 spectrophotometer. Samples with RNA concentration < 20 ng/μL were excluded. For quality control, RNA dilution was performed using Agilent RNA 6000 Nano Kit and 1μL of the sample was run on the Nano chip using an Agilent 2100 electrophoresis bioanalyzer. The quality of total RNA was assessed using the electropherogram’s profile and the calculated RNA integrity number (RIN). All samples showed RINs between 7.3 and 9.3. A total of 6 HD CD8+ and 8 BD CD8+ samples were discarded because of their low RNA concentration or low RIN.Sample Collection - CD8+ T cells were isolated from PBMCs of BD or HD by positive selection using Dynabeads CD8+ isolation kit. Cell purity was ≥ 95%.Labeling - For Illumina Beadarrays, remaining cRNA samples were prepared using Illumina TotalPre-96 RNA Amp kit (LifeTechnologies).MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - Data were further background-corrected using limma R package and interchip batch effects were removed using ComBat method from sva R package.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsDynabeads CD8+ isolation kitRStudio and R with illuminaio, sva and BioMart packagesIllumina TotalPre-96 RNA Amp kitHumanHT-12 V4 beadarrayNucleoSpin RNA kit, NanoDrop ND-1000 and Agilent 2100 bioanalyzertranscription profiling by arrayHomo sapiensPaul RégnierfalseActivated CD8+ T cells through NF-κB signaling pathway are instrumental in Behçet’s syndromeBehçet's disease (BD) is a systemic vasculitis with inflammatory lesions mediated by cytotoxic T cells and neutrophils. Here, we explore the critical involvement of NF-κB signaling pathway in proinflammatory CD8+ T cells differentiation and disease progress of BD patients. Along with other experimental techniques, we performed microarray gene expression analyses of CD8+ T cells from BD patients and HD. Throughout the manuscript, we highlighted that activated CD8+ T cells through NF-κB signaling pathway are instrumental in BD.2026-07-01T00:00:00Z2026-07-01T01:04:26.841Z2026-06-08T15:34:04.08ZE-MTAB-17120EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815