biostudies-arrayexpressMette Ludwig Q.Mus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17123Bulk RNA sequencing of the hippocampus from mice treated with semaglutide or vehicle control prior to lipopolysaccharide (LPS)-induced neuroinflammation.biostudies-arrayexpressSequencing - cDNA libraries were sequenced on a NextSeq 500 using NextSeq 500/550 High Output Kit V2 (Illumina)Sample Collection - Samples were collected and snap-frozen in liquid nitrogen and stored at -80°CNucleic Acid Extraction - RNA was isolated using the NucleoSpin® kit (MACHEREY-NAGEL)Library Construction - A total of 10 ng-1 µg purified RNA from each sample was used to generate a cDNA library using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs)OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw counts are providedSequence Alignment - The bulk RNA-seq data was aligned to the mouse genome obtained from the Ensembl database using the Spliced Transcripts Alignment to a Reference (STAR) softwareMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 500RNA-seq of coding RNAMus musculusSemaglutide Attenuates Neuroinflammation in MiceDylan M. Rausch, Mette Q. Ludwig, Marie A. Bentsen, Stine N. Hansen, Anna Secher, Dorte Holst, Jaime Moreno, Vivek Das, Kristoffer L. Egerod, Anne-Mette Bjerregaard, Kristoffer Niss, Sarah Bau, Charles Pyke, Kevin Dalgaard, Myrte Merkestein, Franziska Wichern, Charlotte Thim Hansen, Joseph Polex-Wolf, Lotte Bjerre Knudsen & Tune H. PersMette Ludwig Q.falseSemaglutide Attenuates Neuroinflammation in MiceBulk RNA sequencing of the hippocampus from mice treated with semaglutide or vehicle control prior to lipopolysaccharide (LPS)-induced neuroinflammation.2026-07-01T00:00:00Z2026-07-01T01:04:21.446Z2026-05-27T13:52:04.098ZE-MTAB-17123ERP194053E-MTAB-16829EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184