biostudies-arrayexpressJoh Demura-DevoreArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17129β-carboline alkaloids produced by Peganum harmala (Syrian rue) have been reported to possess antimicrobial, neurological, and anticancer activities, yet their biological role and molecular target remain unknown. We investigated the effects of the harmala alkaloid, harmaline, on plant growth and cell division using Arabidopsis thaliana as a model system. RNAseq was performed on Arabidopsis seedlings in control, harmaline, and harmine treatments.biostudies-arrayexpressSample Collection - Seedlings were grown on 1/2MS plates with control, harmaline, or harmine treatments.Sequencing - RNA was sequenced at 150-bp PE on an Element AVITI instrument by AmpSeq.Library Construction - Poly-A enriched RNA-seq libraries were prepared by using Vazyme VAHTS Universal V10 RNA-seq Library Prep Kit.Nucleic Acid Extraction - RNA isolation was performed using the Qiagen RNeasy Plant Mini Kit.Growth Protocol - Plants were grown vertically on 1/2 MS 1% sucrose plates in a growth chamber at 22°C under continuous light.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - A pipeline of Trim Galore, HISAT2, samtools, and StringTie was used to determine read count based on raw RNAseq data.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsElement AVITIRNA-seq of coding RNAArabidopsis thalianaJoh Demura-DevorefalseRNAseq of Harmaline and Harmine-treated Arabidopsis thalianaβ-carboline alkaloids produced by Peganum harmala (Syrian rue) have been reported to possess antimicrobial, neurological, and anticancer activities, yet their biological role and molecular target remain unknown. We investigated the effects of the harmala alkaloid, harmaline, on plant growth and cell division using Arabidopsis thaliana as a model system. RNAseq was performed on Arabidopsis seedlings in control, harmaline, and harmine treatments.2026-08-30T00:00:00Z2026-08-30T01:00:47.413Z2026-06-05T17:11:03.179ZE-MTAB-17129ERP194639EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003816EFO_0003738EFO_0004184