biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsSreejith PerinthottathilmicroRNA profiling by arrayDrosophila melanogasterDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17143Expression analysis of microRNAs in 0-1 day old testis in Wildtype vs Mutantsbiostudies-arrayexpressLin28 Represses Hub Cell microRNAs to Maintain Drosophila Testis Niche FunctionSample Collection - 0-1 Day old testis were dissected in PBSNucleic Acid Extraction - Total RNA was extracted using Ambion miRNA extaraction kit according to manufactures protocol. Sample RNA Quality Control was performed using an Agilent 2100 bioanalyzer. The Bioanalyzer provides an electropherogram for each sample (see the RNA_QC.pdf – file) giving a good estimate of RNA integrity. As well as the traditional measurement of the rRNA ratio (28S/18S), the Bioanalyzer provides a RIN value (RNA Integrity Number ranging from 0 to 10), which gives a reliable impression of the quality of the RNA (Schroeder et al, 2006). We normally recommend RIN values higher than 7 for good array performance.Scaning - After hybridization the microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) in order to prevent potential bleaching of the fluorescent dyes. The miRCURY LNA™ microRNA Array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the image analysis was carried out using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon, Denmark). The quantified signals were background corrected (Normexp with offset value 10, see Ritchie et al. 2007) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.Hybridization - The Hy3™-labeled samples and a Hy5™-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURY LNA™ microRNA Array Other Species (Exiqon, Denmark), which contains capture probes targeting all microRNAs for human, mouse or rat registered in the miRBASE 18.0. The hybridization was performed according to the miRCURY LNA™ microRNA Array Instruction manual using a Tecan HS4800™ hybridization station (Tecan, Austria).Labeling - 750 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer.MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsSreejith PerinthottathilSreejith Perinthottathil and Changsoo KimfalsemiRNA profiling of in Drosophila melanogaster TestisExpression analysis of microRNAs in 0-1 day old testis in Wildtype vs Mutants2026-06-23T00:00:00Z2026-06-23T01:00:43.7Z2026-06-10T15:33:18.001ZE-MTAB-17143EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003815