{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["xiao yang"],"instrument_platform":["NextSeq 500"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17157"],"description":["This study contains bulk RNA sequencing datasets generated from THP1-derived macrophages and primary monocyte-derived macrophages under M0 and M1 polarization conditions. Wild-type and TET2-deficient cells were analyzed to characterize transcriptional programs associated with TET2 loss-of-function and phagocytosis-related pathways."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - THP1-derived macrophages and primary monocyte-derived macrophages were cultured under M0 and M1 polarization conditions. Cells were harvested at the indicated experimental endpoints and collected for RNA extraction.","Library Construction - RNA sequencing libraries were prepared from purified total RNA according to the manufacturer's instructions. Libraries were quality controlled and quantified before sequencing.","Sequencing - Paired-end RNA sequencing was performed on an Illumina platform with a read length of 150 bp. Sequencing generated paired FASTQ files for downstream transcriptomic analysis.Paired-end RNA sequencing was performed on an Illumina platform with a read length of 150 bp. Sequencing generated paired FASTQ files for downstream transcriptomic analysis.","Nucleic Acid Extraction - Total RNA was extracted from THP1-derived macrophages and primary monocyte-derived macrophages using a standard RNA isolation procedure. RNA quality and quantity were assessed prior to library preparation."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["xiao yang"],"additional_accession":[]},"is_claimable":false,"name":"Supporting datasets for: Mutation-resolved single-cell transcriptomics reveals enhanced β-amyloid clearance in TET2-mutant human monocytes","description":"This study contains bulk RNA sequencing datasets generated from THP1-derived macrophages and primary monocyte-derived macrophages under M0 and M1 polarization conditions. Wild-type and TET2-deficient cells were analyzed to characterize transcriptional programs associated with TET2 loss-of-function and phagocytosis-related pathways.","dates":{"release":"2026-06-16T00:00:00Z","modification":"2026-06-16T20:16:29.519Z","creation":"2026-06-12T14:50:44.461Z"},"accession":"E-MTAB-17157","cross_references":{"ENA":["ERP194992"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}