biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsxiao yangNextSeq 500RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17157This study contains bulk RNA sequencing datasets generated from THP1-derived macrophages and primary monocyte-derived macrophages under M0 and M1 polarization conditions. Wild-type and TET2-deficient cells were analyzed to characterize transcriptional programs associated with TET2 loss-of-function and phagocytosis-related pathways.biostudies-arrayexpressSample Collection - THP1-derived macrophages and primary monocyte-derived macrophages were cultured under M0 and M1 polarization conditions. Cells were harvested at the indicated experimental endpoints and collected for RNA extraction.Library Construction - RNA sequencing libraries were prepared from purified total RNA according to the manufacturer's instructions. Libraries were quality controlled and quantified before sequencing.Sequencing - Paired-end RNA sequencing was performed on an Illumina platform with a read length of 150 bp. Sequencing generated paired FASTQ files for downstream transcriptomic analysis.Paired-end RNA sequencing was performed on an Illumina platform with a read length of 150 bp. Sequencing generated paired FASTQ files for downstream transcriptomic analysis.Nucleic Acid Extraction - Total RNA was extracted from THP1-derived macrophages and primary monocyte-derived macrophages using a standard RNA isolation procedure. RNA quality and quantity were assessed prior to library preparation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB Filesxiao yangfalseSupporting datasets for: Mutation-resolved single-cell transcriptomics reveals enhanced β-amyloid clearance in TET2-mutant human monocytesThis study contains bulk RNA sequencing datasets generated from THP1-derived macrophages and primary monocyte-derived macrophages under M0 and M1 polarization conditions. Wild-type and TET2-deficient cells were analyzed to characterize transcriptional programs associated with TET2 loss-of-function and phagocytosis-related pathways.2026-06-16T00:00:00Z2026-06-16T20:16:29.519Z2026-06-12T14:50:44.461ZE-MTAB-17157ERP194992EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184