{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Stefano Colombo"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17174"],"description":["Data from our group has shown that the protozoan parasite Trypanosoma brucei brucei can invade the mouse female reproductive tract (FRT) causing changes in tissue architecture and immune profile, as well as inducing reproductive cycle arrest. The purpose of this experiment was to identify transcriptional changes across the hypothalamic–pituitary–gonadal (HPG) axis during infection to link parasite-induced changes in hormonal regulation with the observed phenotype in the FRT. Female C57BL/6J mice were infected with 2x10^3 T. brucei brucei Antat 1.1E parasites via intraperitoneal injection. Mice were euthanised at 7 and 21-24 days post-infection, representing the acute and chronic stages of infection, respectively. Tissue was collected into RNAlater and stored at -80oC prior to RNA extractio"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Female C57BL/6J mice were infected with 2 x 10^3 Trypanosoma brucei brucei parasites via intraperitoneal injection. Mice were euthanised by CO2 in rising concentration at 7 days post infection or 21-24 days post infection representing acute and chronic time points respectively. Control infection naive mice were also collected.   Following euthenisation the ovaries, hypothalamus and pituitary gland were isolated by dissection. Placed in RNAlater and flash-frozen on dry ice. Tissue samples were stored at -70oC prior to RNA isolation.","Library Construction - Libraries were prepared using the Illumina Stranded mRNA Prep. Ligation Kit according to the manufacturer’s protocol","Sequencing - Illumina sequencing was performed on an Illumina NovaSeq6000 machine.","Nucleic Acid Extraction - RNA was isolated by acid-phenol chloroform extraction using TRIzol Reagent (Invitrogen) following the manufacturer's instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Normalization was performed using DESeq2.  Processed 'raw' reads are provided."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["N/A","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_title":["T. brucei infection remodels the uterine immune environment and drives neuroendocrine dysfunction"],"pubmed_authors":["Olivia M. Shorthouse, Chloe Barnes, Stefano A. P. Colombo, Alice H. Costain, Katrine Wonsbek, Anmoyul Mohon, Jessica Costa, Matthew C. Sinton, David J. Thornton, Andrew S. MacDonald, Elizabeth Mann, and Juan F. Quintana","Olivia Shorthouse","Stefano Colombo","Juan Quintana"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of C57BL/6J female mouse ovaries, hypothalamus, and pituitary gland tissue in animals infected with Trypanosoma brucei brucei at acute and chronic time points","description":"Data from our group has shown that the protozoan parasite Trypanosoma brucei brucei can invade the mouse female reproductive tract (FRT) causing changes in tissue architecture and immune profile, as well as inducing reproductive cycle arrest. The purpose of this experiment was to identify transcriptional changes across the hypothalamic–pituitary–gonadal (HPG) axis during infection to link parasite-induced changes in hormonal regulation with the observed phenotype in the FRT. Female C57BL/6J mice were infected with 2x10^3 T. brucei brucei Antat 1.1E parasites via intraperitoneal injection. Mice were euthanised at 7 and 21-24 days post-infection, representing the acute and chronic stages of infection, respectively. Tissue was collected into RNAlater and stored at -80oC prior to RNA extractio","dates":{"release":"2026-09-07T00:00:00Z","modification":"2026-09-07T08:33:33.669Z","creation":"2026-06-16T21:10:27.849Z"},"accession":"E-MTAB-17174","cross_references":{"ENA":["ERP195218"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}