{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Carl MANN"],"organism":["Homo sapiens"],"software":["Salmon, tximeta, DESeq2, limma, voom"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17208"],"description":["Two independent Crisr/Cas9 knock-out mutants in the WI38hTERT human fibroblast cell line were generated for RNA-seq analysis to assess the role of FOXF1 in inflammatory gene expression during X-ray induced senescence."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Paired-End 42 nucleotides in an Illumina NextSeq 500 sequencer.","Growth Protocol - DMEM + glutamine + pyruvate + pen/strep in 5% O2 + 5% CO2 incubator.","Nucleic Acid Extraction - RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus.","Sample Collection - Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer.","Sample Treatment - 40 Gy X-rays at 120 kV generated by a Faxitron 43855-D generator","Library Construction - NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selection"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - Fastq sequences were mappled to the human transcriptome using the Salmon transcriptome alignment software."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 550"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Carl MANN"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of FOXF1-KO mutants in the WI38hTERT cell line in proliferation and in senescence induced by ionizing irradiation","description":"Two independent Crisr/Cas9 knock-out mutants in the WI38hTERT human fibroblast cell line were generated for RNA-seq analysis to assess the role of FOXF1 in inflammatory gene expression during X-ray induced senescence.","dates":{"release":"2026-06-26T00:00:00Z","modification":"2026-06-26T01:00:44.247Z","creation":"2026-06-16T16:57:52.983Z"},"accession":"E-MTAB-17208","cross_references":{"ENA":["ERP195212"],"Biostudies":["E-MTAB-17100","E-MTAB-16030","E-MTAB-14265","E-MTAB-17105","E-MTAB-14201"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0004917","EFO_0005518","EFO_0003738","EFO_0004184","EFO_0003969"]}}