biostudies-arrayexpressMark CarringtonTrypanosoma brucei bruceihttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17235Most protozoan pathogens of mammals are transmitted by invertebrate vectors and a comparison of transcriptomes of the developmental forms provides information on genes involved in interactions with different hosts. Trypanosoma brucei is the causal agent of African Trypanosomiasis and is transmitted by Tsetse flies. Here, cultured mammalian bloodstream forms were differentiated in vitro to insect procyclic forms and RNAseq experiments performed on the two developmental forms.biostudies-arrayexpressSequencing - Sequencing performed by the Beijing Genomics Institute using BGI DNBSEQ Eukaryotic Strand-specific Transcriptome Resequencing protocol Read Length PE 150bpSample Collection - Cells grown In culture were harvest by centrifugation, washed once, and dissolved in RNA lysis solution within 4 minutesNucleic Acid Extraction - Qiagen RNAeasy mini kit to prepare total RNA which was shipped to the Beijing Genomics InstituteLibrary Construction - Library construction performed by the Beijing Genomics Institute using BGI DNBSEQ Eukaryotic Strand-specific Transcriptome Resequencing protocolOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMetabolomicsUnknownTranscriptomicsGenomicsProteomicsDNBSEQ-G50RNA-seq of coding RNATrypanosoma brucei bruceiTranscriptomes from Trypanosoma brucei brucei EATRO1125 before and after differentiation of bloodstream forms to procyclic forms.Fumiko Kawasaki, Janaina de Freitas Nascimento and Mark CarringtonMark CarringtonfalseTranscriptomes from Trypanosoma brucei brucei EATRO1125 before and after differentiation of bloodstream forms to procyclic formsMost protozoan pathogens of mammals are transmitted by invertebrate vectors and a comparison of transcriptomes of the developmental forms provides information on genes involved in interactions with different hosts. Trypanosoma brucei is the causal agent of African Trypanosomiasis and is transmitted by Tsetse flies. Here, cultured mammalian bloodstream forms were differentiated in vitro to insect procyclic forms and RNAseq experiments performed on the two developmental forms.2026-07-11T00:00:00Z2026-07-11T01:00:48.732Z2026-06-29T13:17:04.983ZE-MTAB-17235ERP195881EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184