biostudies-arrayexpressWendy Le MouëllicMycobacterium tuberculosishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17251RNA-seq analysis of total RNA was conducted on wild-type Mycobacterium tuberculosis (H37Rv strain) grown in presence of 0, 20 or 100µM copper to uncover transcriptomic adaptations resulting from the exposure to this metal.biostudies-arrayexpressLibrary Construction - Each total RNA sample was barcoded with CEL-seq primers during enzymatic lysis, followed by fragmentation, end repair, poly(A) tailing, reverse transcription, and second-strand synthesis. After second-strand synthesis, barcoded samples were pooled into a single library and amplified by in vitro transcription. Ribosomal amplified RNA was then depleted, after which adapter ligation, reverse transcription, and indexing PCR were performed to generate the final cDNA library.Nucleic Acid Extraction - Total bacterial RNA was extracted from 10 mL cultures harvested at logarithmic phase (OD600 = 0.8) using the RNeasy kit (Qiagen) and treated with DNase I (Thermo Scientific).Growth Protocol - Mycobacterium tuberculosis H37Rv bacteria were grown in triplicate in modified copper-free 7H9 medium supplemented with either no added copper, 20 µM CuSO4, or 100 µM CuSO4 until OD600=0.8.Sequencing - Libraries were sequenced in paired-end mode on an Illumina NovaSeq X Plus platform (read 1, 26 cycles; index read, 6 cycles; read 2, 60 cycles).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Raw sequencing reads were processed with Cutadapt v4.9 to remove adapter sequences and homopolymers, then filtered with Ribodetector v0.3.1 to remove ribosomal reads. Processed reads were aligned to the M. tuberculosis H37Rv reference genome (assembly ASM19595v2) using STARsolo v2.7.11b with the parameter --soloFeatures GeneFull_Ex50pA.Data Transformation - The processed file 'raw_counts_all_samples' contains the unnormalized raw read counts for all mapped genes across each sample.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of total RNAMycobacterium tuberculosisYannick PoquetWendy Le MouëllicOlivier NeyrollesFlorence LevillainfalseRNA-seq of wild-type H37Rv strain of Mycobacterium tuberculosis exposed to 0, 20 or 100µM copperRNA-seq analysis of total RNA was conducted on wild-type Mycobacterium tuberculosis (H37Rv strain) grown in presence of 0, 20 or 100µM copper to uncover transcriptomic adaptations resulting from the exposure to this metal.2026-08-25T00:00:00Z2026-08-25T09:09:44.929Z2026-06-29T15:00:26.331ZE-MTAB-17251ERP195889EFO_0002944EFO_0004170EFO_0009653EFO_0003789EFO_0004917EFO_0003816EFO_0004184