{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Nicole Leonie Bertschi"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17266"],"description":["Effect of Tapinaorf on normal human keratinocytes (NHEK). NHEKs were e treated with tapinarof (1uM) or DMSO (control) for 72 hours."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Normal human epidermal keratinocyte (NHEK) (Cat. C-12003, Promocell) vials from different donors were obtained and cultured in Dermacult medium (Cat. 100-0500, Stemcell Technologies) containing Keratinocyte Expansion Supplement (Cat. 100-0500, Stemcell Technologies), Hydrocortisone (Cat. 07925, Stemcell Technologies), penicillin (50 U/ml) and streptomycin (50 μg/ml) (Cat. 4-01F00-H, BioConcept). Keratinocytes were cultured in T75 flasks and medium was exchanged every other day. After reaching 90% confluency, keratinocytes were washed with D-PBS (Cat. 37354, Stemcell Technologies) and treated with ACCUTASE (Cat. 07922, Stemcell Technologies). After 5-7 minutes at 37°C, keratinocyte were dislodged with gentle tapping of the plate, transferred to a 15ml conical tube and wa","Library Construction - Illumina TruSeq Stranded mRNA protocol (Directional, first strand)","Sequencing - The mRNA was sequenced by the Next Generation Sequencing Platform of Bern using paired-end 100 bp sequencing.","Nucleic Acid Extraction - The RNeasy Micro Kit (Cat. 74004, Qiagen) was used for total RNA isolation according to manufacturer’s instructions"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Nicole Leonie Bertschi"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of normal human keratinocytes (NHEK) treated with tapinarof against untreated control","description":"Effect of Tapinaorf on normal human keratinocytes (NHEK). NHEKs were e treated with tapinarof (1uM) or DMSO (control) for 72 hours.","dates":{"release":"2026-07-10T00:00:00Z","modification":"2026-07-10T01:00:54.707Z","creation":"2026-07-01T16:03:46.756Z"},"accession":"E-MTAB-17266","cross_references":{"ENA":["ERP195978"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}