biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNicole Leonie BertschiIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17266Effect of Tapinaorf on normal human keratinocytes (NHEK). NHEKs were e treated with tapinarof (1uM) or DMSO (control) for 72 hours.biostudies-arrayexpressSample Collection - Normal human epidermal keratinocyte (NHEK) (Cat. C-12003, Promocell) vials from different donors were obtained and cultured in Dermacult medium (Cat. 100-0500, Stemcell Technologies) containing Keratinocyte Expansion Supplement (Cat. 100-0500, Stemcell Technologies), Hydrocortisone (Cat. 07925, Stemcell Technologies), penicillin (50 U/ml) and streptomycin (50 μg/ml) (Cat. 4-01F00-H, BioConcept). Keratinocytes were cultured in T75 flasks and medium was exchanged every other day. After reaching 90% confluency, keratinocytes were washed with D-PBS (Cat. 37354, Stemcell Technologies) and treated with ACCUTASE (Cat. 07922, Stemcell Technologies). After 5-7 minutes at 37°C, keratinocyte were dislodged with gentle tapping of the plate, transferred to a 15ml conical tube and washed with the keratinocyte medium. For the treatment, keratinocytes were plated into a 12 well plate at a density of 1x 105 cells per well and cultured for 24h in keratinocyte medium. After 24h, the medium was exchanged to keratinocyte medium containing 1.2mM CaCl2 (Cat. C34006, Sigma-Aldrich) and NHEKs were treated with DMSO or tapinarof (1 μM. at. #S9700, Selleck Chemicals). NHEKs were harvested after 3 days of treatmentLibrary Construction - Illumina TruSeq Stranded mRNA protocol (Directional, first strand)Sequencing - The mRNA was sequenced by the Next Generation Sequencing Platform of Bern using paired-end 100 bp sequencing.Nucleic Acid Extraction - The RNeasy Micro Kit (Cat. 74004, Qiagen) was used for total RNA isolation according to manufacturer’s instructionsOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesNicole Leonie BertschifalseRNA-seq of normal human keratinocytes (NHEK) treated with tapinarof against untreated controlEffect of Tapinaorf on normal human keratinocytes (NHEK). NHEKs were e treated with tapinarof (1uM) or DMSO (control) for 72 hours.2026-07-10T00:00:00Z2026-07-10T01:00:54.707Z2026-07-01T16:03:46.756ZE-MTAB-17266ERP195978EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184